LOCUS       sequence-111112-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Sp dCas9-3XGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-TO-dCas9-3XGFP
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..754)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 tgtaatccag aggttgatta tctagattac cctactcgag tttgtacagt tcatccatac
       61 catgcgtgat gcccgctgcg gttacgaact ccagcagaac catatgatcg cgtttctcgt
      121 tcggatcttt agacagaacg ctttgcgtgc tcagatagtg attgtctggc agcagaacag
      181 gaccatcacc gattggagtg ttttgctggt agtgatcagc cagctgcacg ctgccatcct
      241 ccacgttgtg gcgaatttta aaattcgctt taatgccatt tttttgttta tcggcggtga
      301 tgtaaacatt gtggctgtta aaattgtatt ccagcttatg gcccaggata ttgccgtctt
      361 ctttaaagtc aatgcctttc agctcaatgc ggtttaccag ggtatcgcct tcaaatttca
      421 cttccgcacg cgttttgtac gtgccgtcat ccttaaagga aatcgtgcgt tcctgcacat
      481 agccttccgg catggcggac ttgaagaagt catgctgctt catatggtcc ggataacgag
      541 caaagcactg aacaccataa gtcagcgtcg ttaccagagt cggccaaggt accggcagtt
      601 taccagtagt acagatgaac ttcagcgtca gtttaccatt agttgcgtca ccttcaccct
      661 cgccacgcac ggaaaactta tgaccgttga catcaccatc cagttccacc agaataggga
      721 cgacaccagt gaacagctct tcgcctttac gcatgctacc atcgatgcta cctttgtaca
      781 gttcatccat accatgcgtg atgcccgctg cggttacgaa ctccagcaga accatatgat
      841 cgcgtttctc gttcggatct ttagacagaa cgctttgcgt gctcagatag tg
//
