LOCUS       sequence-111115-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast recombinational cloning compatible Agrobacterium tumefaciens 
            ternary vector containing bar gene (for resistance against 
            glufosinate ammonium) on transfer DNA (TDNA)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Sidhu YS, Cairns TC, Chaudhari YK, Usher J, Talbot NJ, Studholme DJ,
            Csukai M, Haynes K
  TITLE     Exploitation of sulfonylurea resistance marker and non-homologous 
            end joining mutants for functional analysis in Zymoseptoria tritici.
  JOURNAL   Fungal Genet Biol. 2015 Jun;79:102-9. doi: 
            10.1016/j.fgb.2015.04.015.
  PUBMED    26092796
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2015-06"; volume: "79:102-9. doi"; pages: " 
            10.1016/j.fgb.2015.04.015"
COMMENT     Sequence Label: pC-BAR-YR
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..28)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(137..688)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
ORIGIN
        1 tgtattacac gtggtggtgg tggtggtggc tagcgttaac actagtcaga tctaccatgg
       61 tggactcctc ttaaagcttg catgcctgca ggtcgactct agaggatccc caactggttc
      121 ccggtcggca tctactctaa atctcggtga cgggcaggac cggacggggc ggaaccggca
      181 ggctgaagtc cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg
      241 cccgcagcat gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt
      301 tgggcagccc gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca
      361 ggtgggtgta gagcgtggag cccagtcccg tccgctggtg gcggggggag acgtacacgg
      421 tcgattcggc cgtccagtcg taggcgttgc gtgccttcca gggacccgcg taggcgatgc
      481 cggcgacctc gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga
      541 ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct
      601 cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga
      661 tgtcggccgg gcgtcgttct gggctcattt ggatgcttgg gtagaatagg taagtcagat
      721 tgaatctgaa ataaagggag gaagggcgaa cttaagaagg tatgaccggg tcgtccactt
      781 accttgcttg acaaacgcac caagttatcg tgcaccaagc agcagatgat aataatgtcc
      841 tcgttcctgt ctgctaataa
//
