LOCUS       sequence-111119-         573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 573)
  TITLE     SGC Toronto Q4 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4RRE
FEATURES             Location/Qualifiers
     source          1..573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             12..29
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             51..71
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      228..275
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 tgtccagcgg tctggttccg cgtggttccg gtaccgcggc ccagccggcc gaaaatctgt
       61 acttccaagg tcgtcgtgcc tctgtggagc tcccgctggg tccgctgccg ccgggttggg
      121 aaaaacgcac cgatagcaat ggtcgtgtct actttgtcaa tcataatacg cgcattacgc
      181 agtgggaaga tccgcgtagc cagggccaat aggcggccgc ataataaacc gatacaatta
      241 aaggctcctt ttggagcctt ttttttggag attttcaacg tgaaaaaatt attattcgca
      301 attcctttag ttgttccttt ctattctcac tccgctgaaa ctgttgaaag ttgtttagca
      361 aaaccccata cagaaaattc atttactaac gtctggaaag acgacaaaac tttagatcgt
      421 tacgctaact atgagggctg tctgtggaat gctacaggcg ttgtagtttg tactggtgac
      481 gaaactcagt gtctagctag agtggcggtg gctctggttc cggtgatttt gattatgaaa
      541 agatggcaaa cgctaataag ggggctatga ccg
//
