LOCUS       sequence-111123-         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of St1 dCas9-3XTagBFP2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6..24
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    27..45
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     55..79
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      172..181
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             184..204
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agctctccct atcagtgata gagatctccc tatcagtgat agagatcgtc gacgagctcg
       61 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
      121 acaccgggac cgatccagcc taccggttaa ggtgaacgcg tgcaggctgg cgccaccatg
      181 gcccctaaaa agaaacgcaa ggtcgaattc ggaagcgacc tggtgctggg cctggccatc
      241 ggcatcggca gcgtgggcgt gggcatcctg aacaaggtga ccggcgagat catccacaag
      301 aacagtcgca tcttccctgc tgctcaggct gagaacaacc tggtgcgccg caccaaccgc
      361 cagggtcgcc ggcttgctcg ccgcaagaag caccggcgcg tgcgcctgaa ccgcctgttc
      421 gaggagagcg gcctgatcac cgacttcacc aagatcagca tcaacctgaa cccctaccag
      481 ctgcgcgtga agggcctgac cgacgagctg agcaacgagg agctgttcat cgccctgaag
      541 aacatggtga agcaccgcgg catcagctac ctggacgacg ccagcgacga cggcaacagc
      601 agcgtgggcg actacgccca gatcgtgaag gagaacagca agcagctgga gaccaagacc
      661 cccggccaga tccagctgga gcgctacca
//
