LOCUS       sequence-111131-         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  TITLE     SGC Toronto Q4 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4RXJ
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..63
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             429..446
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(499..517)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      513..560
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 agatatacca tgcatcatca tcatcatcac agcagcggca gagaaaactt gtatttccag
       61 ggcaaagctg gcaagaaact acattacaag cagattgttt gggtcaaatt gggaaattac
      121 agatggtggc cagcagagat ctgcaacccc aggtctgtgc cactgaacat ccagggcctt
      181 aaacatgact tgggggactt ccctgtattc ttctttggtt ctcatgacta ctactgggta
      241 caccagggca gagtgttccc ttatgttgaa ggagacaaaa gctttgctga agggcagact
      301 agtattaaca agaccttcaa aaaggcactg gaagaagctg caaaacgttt ccaggaattg
      361 aaagcacaaa gagaaagtaa agaagcccta gagattgaat gatgacgaag cttgcggccg
      421 cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
      481 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      541 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc
      601 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
      661 acttgccagc g
//
