LOCUS       sequence-111133-         575 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 575)
  TITLE     SGC Toronto Q4 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4ROJ
FEATURES             Location/Qualifiers
     source          1..575
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..63
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             441..458
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(511..529)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      525..572
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 agatatacca tgcatcatca tcatcatcac agcagcggca gagaaaactt gtatttccag
       61 ggcgactaca ctgcataccc ctggtttgca ggtaacatgg agaggcagca gacggacaac
      121 ctgctcaagt cccacgccag cgggacctac ctgatcaggg agcggcctgc cgaggctgag
      181 cgctttgcaa taagcatcaa gttcaatgat gaggtgaagc acatcaaggt ggtggagaag
      241 gacaactgga tccacatcac agaggccaag aaattcgaca gcctcctgga gttggtggag
      301 tactaccagt gccactcact gaaggagagc ttcaagcagc tggacaccac actcaagtac
      361 ccctacaagt cccgggaacg ttcggcctcc agggcctcca gccggtcccc atgatgacga
      421 agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag
      481 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      541 gggcctctaa acgggtcttg aggggttttt tgctg
//
