LOCUS       sequence-111138-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Xia Z, Webster A, Du F, Piatkov K, Ghislain M, Varshavsky A
  TITLE     Substrate-binding sites of UBR1, the ubiquitin ligase of the N-end 
            rule pathway.
  JOURNAL   J Biol Chem. 2008 Aug 29. 283(35):24011-28.
  PUBMED    18566452
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Aug 29. 283(35):24011-28."
COMMENT     Sequence Label: pFLAG UBR1 SBX
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(18..265)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     231..249
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     protein_bind    321..332
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 agcttgcatg cctgcaggca aattaaagcc ttcgagcgtc ccaaaacctt ctcaagcaag
       61 gttttcagta taatgttaca tgcgtacacg cgtctgtaca gaaaaaaaag aaaaatttga
      121 aatataaata acgttcttaa tactaacata actataaaaa aataaatagg gacctagact
      181 tcaggttgtc taactccttc cttttcggtt agagcggatg tggggggagg gcgtgaatgt
      241 aagcgtgaca taactaatta catgatctcg agggatccct accaaatctc tcgctcatca
      301 gagtcatcat tatcatcaga gtcatcctca tcgaggtttc ttggatttgt atttcttatg
      361 atagtagctc catcaaattg aggtcctaag aactgcaata ttggtggggg attctgaggt
      421 gcatcatcat cgccatttcc ctgcggttgt agagtgttcc taaagttttc gaagaattgg
      481 aaaaaatcac gtactccgcc accagcaata ccttctgcgc caaccccaaa taagttggcg
      541 gcctgtccag tttcatcatc aacatccatt tcgtcatttc cctccgtaaa aaacccgtct
      601 tctccctctt ccatatcttc atcatcttca tctgttggag gtattcttct tggccttggt
      661 tctctattaa atgcaaacaa gaaaccattg gaaagtatag tcactcgaaa ttcgtcaccc
      721 attactctac taatataacc tggaatttca ttgttaatcc ataatctgtt taagtgctca
      781 tatcttttca aattcaaggt ggttaaatca cctcttctca tggcatttct accaacttca
      841 ccatgcga
//
