LOCUS       sequence-111140-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast recombinational cloning compatible Agrobacterium tumefaciens 
            ternary vector containing nptII (neomycin phosphotransferase II) 
            selectable marker on transfer DNA (TDNA)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Sidhu YS, Cairns TC, Chaudhari YK, Usher J, Talbot NJ, Studholme DJ,
            Csukai M, Haynes K
  TITLE     Exploitation of sulfonylurea resistance marker and non-homologous 
            end joining mutants for functional analysis in Zymoseptoria tritici.
  JOURNAL   Fungal Genet Biol. 2015 Jun;79:102-9. doi: 
            10.1016/j.fgb.2015.04.015.
  PUBMED    26092796
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2015-06"; volume: "79:102-9. doi"; pages: " 
            10.1016/j.fgb.2015.04.015"
COMMENT     Sequence Label: pC-G418-YR
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..27)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(510..529)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tattcacacg tggtggtggt ggtggtggct agcgttaaca ctagtcagat ctaccatggt
       61 ggactcctct taaagcttgc atgcctgcag gtcgactcta gaggatcccc aactggttcc
      121 cggtcggcat ctactatcat catgcaacat gcatgtactg tctgatgtat taagagtata
      181 ggggtcaaca actagagtgt ctgacaggta tatctagcag tttgatttga ccattctttt
      241 attaaaacac tgtaacccgc aacgaagaac tcctccgctt caagtcaaga atgcgcatat
      301 gtgcgtaaaa tcgacaagcc cggcggcatt ccaccgagca gggtattgcc tttggcactt
      361 tacgtgcgca gaggagcctg aatgttgagt ggaatgattc agaagaactc gtcaagaagg
      421 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
      481 tcagcccatt cgccgccaag ttcttcagca atatcacggg tagccaacgc tatgtcctga
      541 tagcggtccc ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
      601 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
      661 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ttcttcgtcc
      721 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
      781 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
      841 tcagccatga tggatacttt c
//
