LOCUS       sequence-111143-         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 1-3 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX1-3
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(400..417)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(400..416)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(409..431)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      480..566
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgtcacccgg caaccttggg tagcagcgaa gtcgaggcat ttctgtcctg gctggtctag
       61 acgtctcgtc aggccatggt cctggcacag caccggcaac agccgctgca ccgtttcgag
      121 cgcttgcttg ccgccattgt tgctggcgat agccaccact tggtccgggg tcaggccatg
      181 gtcctggcac agcaccggca acagccgctg caccgtttcg agcgcttgct tgccgccaat
      241 gttgctggcg atagccacca cttggtccgg ggtcaggcca tggtcctggc acagcaccgg
      301 caacagccgc tgcaccgttt cgagcgcttg cttgccgcca atgttgctgg cgatagccac
      361 cacttggtcc ggggtcaggt tcagggggag acgcttaaga ctggccgtcg ttttacaacg
      421 tcgtgactgg gaaaacatcc atgctagcgt taacgcgaga gtagggaact gccaggcatc
      481 aaataaaacg aaaggctcag tcggaagact gggcctttcg ttttatctgt tgtttgtcgg
      541 tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gtgaagcaac
      601 ggcccggaag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aact
//
