LOCUS       sequence-111166-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5′ TOP TRICK reporter mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer 
            RH, Chao JA
  TITLE     Translation. An RNA biosensor for imaging the first round of 
            translation from single cells to living animals.
  JOURNAL   Science. 2015 Mar 20;347(6228):1367-671. doi: 
            10.1126/science.aaa3380.
  PUBMED    25792328
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-03-20- 20"; volume: "347"; 
            issue: "6228"; pages: "1367-671"
COMMENT     Sequence Label: pSF4 CMV 5TOP intron renilla TRICK CTE polyA
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          94..226
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     209..228
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        271..289
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      293..302
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcctatataa gcagagctcg tttaggccac cgtcctctct tcctcggcgc tgcctacgga
       61 ggtggcagcc atctccttct cggcaccggt caggtaagta tcaaggttac aagacaggtt
      121 taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
      181 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt ccactcccag
      241 ttcaattaca gctcttaagg ctagagtact taatacgact cactataggc tagccaccat
      301 ggcttccaag gtgtacgacc ccgagcaacg caaacgcatg atcactgggc ctcagtggtg
      361 ggctcgctgc aagcaaatga acgtgctgga ctccttcatc aactactatg attccgagaa
      421 gcacgccgag aacgccgtga tttttctgca tggtaacgct gcctccagct acctgtggag
      481 gcacgtcgtg cctcacatcg agcccgtggc tagatgcatc atccctgatc tgatcggaat
      541 gggtaagtcc ggcaagagcg ggaatggctc atatcgcctc ctggatcact acaagtacct
      601 caccgcttgg ttcgagctgc tgaaccttcc aaagaaaatc atctttgtgg gccacgactg
      661 gggggcttgt ctggcctttc actactccta cgagcaccaa gacaagatca aggccatcgt
      721 ccatgctgag agtgtcgtgg acgtgatcga gtcctgggac gagtggcctg acatcgagga
      781 ggatatcgcc ctgatcaaga gcgaagaggg cgagaaaatg gtgcttgaga ataacttctt
      841 cgtcgagacc atgctcccaa gc
//
