LOCUS       sequence-111171-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to label reporter mRNAs. Lentiviral expression of the MCP 
            protein fused to tagRFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer 
            RH, Chao JA
  TITLE     Translation. An RNA biosensor for imaging the first round of 
            translation from single cells to living animals.
  JOURNAL   Science. 2015 Mar 20;347(6228):1367-671. doi: 
            10.1126/science.aaa3380.
  PUBMED    25792328
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-03-20- 20"; volume: "347"; 
            issue: "6228"; pages: "1367-671"
COMMENT     Sequence Label: phage UBC NLS-HA-2XMCP-tagRFPt
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             70..90
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             94..120
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             94..120
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             121..132
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggcggcc
       61 gcaatgggcc caaaaaagaa aagaaaagtt ggctacccct acgacgtgcc cgactacgcc
      121 atcgaaggcc gccatatgct agccgttaaa atggcttcta actttactca gttcgttctc
      181 gtcgacaatg gcggaactgg cgacgtgact gtcgccccaa gcaacttcgc taacgggatc
      241 gctgaatgga tcagctctaa ctcgcgttca caggcttaca aagtaacctg tagcgttcgt
      301 cagagctctg cgcagaatcg caaatacacc atcaaagtcg aggtgcctaa aggcgcctgg
      361 cgttcgtact taaatatgga actaaccatt ccaattttcg ccacgaattc cgactgcgag
      421 cttattgtta aggcaatgca aggtctccta aaagatggaa acccgattcc ctcagcaatc
      481 gcagcaaact ccggcatcta cgccatggct tctaacttta ctcagttcgt tctcgtcgac
      541 aatggcggaa ctggcgacgt gactgtcgcc ccaagcaact tcgctaacgg gatcgctgaa
      601 tggatcagct ctaactcgcg ttcacaggct tacaaagtaa cctgtagcgt tcgtcagagc
      661 tctgcgca
//
