LOCUS       sequence-111175-         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to label reporter mRNAs. Lentiviral expression of the MCP 
            protein fused to HALO..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer 
            RH, Chao JA
  TITLE     Translation. An RNA biosensor for imaging the first round of 
            translation from single cells to living animals.
  JOURNAL   Science. 2015 Mar 20;347(6228):1367-671. doi: 
            10.1126/science.aaa3380.
  PUBMED    25792328
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-03-20- 20"; volume: "347"; 
            issue: "6228"; pages: "1367-671"
COMMENT     Sequence Label: phage ubc nls ha 2xmcp HALO
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             72..92
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             96..122
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             96..122
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             123..134
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggcgg
       61 ccgcaatggg cccaaaaaag aaaagaaaag ttggctaccc ctacgacgtg cccgactacg
      121 ccatcgaagg ccgccatatg ctagccgtta aaatggcttc taactttact cagttcgttc
      181 tcgtcgacaa tggcggaact ggcgacgtga ctgtcgcccc aagcaacttc gctaacggga
      241 tcgctgaatg gatcagctct aactcgcgtt cacaggctta caaagtaacc tgtagcgttc
      301 gtcagagctc tgcgcagaat cgcaaataca ccatcaaagt cgaggtgcct aaaggcgcct
      361 ggcgttcgta cttaaatatg gaactaacca ttccaatttt cgccacgaat tccgactgcg
      421 agcttattgt taaggcaatg caaggtctcc taaaagatgg aaacccgatt ccctcagcaa
      481 tcgcagcaaa ctccggcatc tacgccatgg cttctaactt tactcagttc gttctcgtcg
      541 acaatggcgg aactggcgac gtgactgtcg ccccaagcaa cttcgctaac gggatcgctg
      601 aatggatcag ctctaactcg cgttcacagg cttacaaagt aacctgtagc gttcgtcaga
      661 gctctgcgca
//
