LOCUS       sequence-111179-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Initial Integrative Vector
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, 
            Patron-Soberano A, De Las Penas A, Castano I
  TITLE     Expression vectors for C-terminal fusions with fluorescent proteins 
            and epitope tags in Candida glabrata.
  JOURNAL   Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020.
  PUBMED    25986172
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020."
COMMENT     Sequence Label: pYC44
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..46
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     30..46
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(48..81)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        411..754
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 aattggagct ccaccgcggt ggcggccgct ctagaactag tggatccgaa gttcctatac
       61 tttctagaga ataggaactt cgtgcgcttt tgaaccacgt aaagtgctgt aaaccagctc
      121 tattcaatga ggtaaatgat aaagtttata agagaccaaa aaattcaaaa aaaaaattga
      181 aaaattatat tggtatttat atgaaacaat agttcagttc aaagcgctag cttttctcat
      241 ctcaactaac cggtattagg tgggtattta tttaatatgg gaacaagaaa tgtgacatta
      301 atgttcatgt ttttattttc attatttatt ctatatggtt tgggacttga tgttatgtct
      361 taattcatag agatccgctt gcctcgtccc cgccgggtca cccggccagc gacatggagg
      421 cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc
      481 gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata cattttgatg
      541 gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg
      601 ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag
      661 gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat
      721 acagttctca catcacatcc gaacataaac aaccggatca tcctacaatt atgtctacta
      781 ctttggatga tactgcttat agatacagaa cttctgttcc aggtgatgct gaagctattg
      841 aagctttgga tggttctttc actaccgata ctgtt
//
