LOCUS       sequence-111185-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recipient integrative vector to construct HA tagged fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, 
            Patron-Soberano A, De Las Penas A, Castano I
  TITLE     Expression vectors for C-terminal fusions with fluorescent proteins 
            and epitope tags in Candida glabrata.
  JOURNAL   Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020.
  PUBMED    25986172
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020."
COMMENT     Sequence Label: pYC48
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..54
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     38..54
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             58..147
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    complement(166..199)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 atagggcgaa ttggagctcc accgcggtgg cggccgctct agaactagtg gatcctatac
       61 ccatacgatg ttcctgacta tgcgggctat ccgtatgacg tcccggacta tgcaggctcc
      121 tatccatatg acgttccaga ttacgctgct cagtgctgaa gatccgaagt tcctatactt
      181 tctagagaat aggaacttcg tgcgcttttg aaccacgtaa agtgctgtaa accagctcta
      241 ttcaatgagg taaatgataa agtttataag agaccaaaaa attcaaaaaa aaaattgaaa
      301 aattatattg gtatttatat gaaacaatag ttcagttcaa agcgctagct tttctcatct
      361 caactaaccg gtattaggtg ggtatttatt taatatggga acaagaaatg tgacattaat
      421 gttcatgttt ttattttcat tatttattct atatggtttg ggacttgatg ttatgtctta
      481 attcatagag atccgcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc
      541 cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc
      601 ccgtacattt agcccataca tccccatgta taatcatttg catccataca ttttgatggc
      661 cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga cctgcgagca gggaaacgct
      721 cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt
      781 taggatttgc cactgaggtt cttctttcat atacttcctt
//
