LOCUS       sequence-111187-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recipient integrative vector to construct YFP tagged fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, 
            Patron-Soberano A, De Las Penas A, Castano I
  TITLE     Expression vectors for C-terminal fusions with fluorescent proteins 
            and epitope tags in Candida glabrata.
  JOURNAL   Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020.
  PUBMED    25986172
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020."
COMMENT     Sequence Label: pYC54
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..54
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     38..54
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(88..116)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     711..731
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(781..814)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 atagggcgaa ttggagctcc accgcggtgg cggccgctct agaactagtg gatcctaatg
       61 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      121 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      181 cttaccctta aattgatttg cactactgga aaactacctg ttccatggcc aacacttgtc
      241 actactttgg gttatggtct aatgtgcttt gctagatacc cagatcatat gaaacggcat
      301 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
      361 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
      421 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
      481 gaatacaact ataactcaca caatgtatac atcactgcag acaaacaaaa gaatggaatc
      541 aaagctaact tcaaaattag acacaacatt gaagatggag gtgttcaact agcagaccat
      601 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
      661 tcctatcaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
      721 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa atagagatcc
      781 gaagttccta tactttctag agaataggaa cttcgtgcgc tt
//
