LOCUS       sequence-111188-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recipient replicative vector to construct MYC tagged fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, 
            Patron-Soberano A, De Las Penas A, Castano I
  TITLE     Expression vectors for C-terminal fusions with fluorescent proteins 
            and epitope tags in Candida glabrata.
  JOURNAL   Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020.
  PUBMED    25986172
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020."
COMMENT     Sequence Label: pYC66
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..520
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     primer_bind     complement(630..650)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(630..648)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(669..685)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(669..685)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(682..704)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    693..709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(717..723,724..741,742..747))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    762..783
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gatcctaggg ttaattaacg gtgaacaaaa gctaatctcc gaggaagact tgaacggtga
       61 acaaaaatta atctcagaag aagacttgaa cggactcgac ggtgaacaaa agttgatttc
      121 tgaagaagat ttgaacggtg aacaaaagct aatctccgag gaagacttga acggtgaaca
      181 aaaattaatc tcagaagaag acttgaacgg actcgacggt gaacaaaagt tgatttctga
      241 agaagatttg aacggtgaac aaaagctaat ctccgaggaa gacttgaacg gtgaacaaaa
      301 attaatctca gaagaagact tgaacggact cgacggtgaa caaaagttga tttctgaaga
      361 agatttgaac ggtgaacaaa agctaatctc cgaggaagac ttgaacggtg aacaaaaatt
      421 aatctcagaa gaagacttga acggactcga cggtgaacaa aagttgattt ctgaagaaga
      481 tttgaacggt gaacaaaagc taatctccga ggaagacttg aacggtgaac aaaaattaat
      541 caatcactag ctagtgaatt cgcgccagat ccgaagttcc tatactttct agagcggccg
      601 ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
      661 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      721 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      781 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgc
//
