LOCUS       sequence-111212-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  eukaryotic expression plasmid for VZV glycoprotein L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Birlea M, Owens GP, Eshleman EM, Ritchie A, Traktinskiy I, Bos N, 
            Seitz S, Azarkh Y, Mahalingam R, Gilden D, Cohrs RJ
  TITLE     Human anti-varicella-zoster virus (VZV) recombinant monoclonal 
            antibody produced after Zostavax immunization recognizes the gH/gL 
            complex and neutralizes VZV infection.
  JOURNAL   J Virol. 2013 Jan;87(1):415-21. doi: 10.1128/JVI.02561-12. Epub 2012
            Oct 17.
  PUBMED    23077312
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02561-12"; journalName: "J Virol"; date: "2013-01"; 
            volume: "87"; issue: "1"; pages: "415-21"
COMMENT     Sequence Label: VZV ORF 60 (gL)
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(44..61)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(550..568)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(611..630)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          613..745
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 agcattaccc tcactaaagg gaagcggccg ctcagtcgac tcaatgatgg tggtgatggt
       61 gctcgagttg gcatacgcgt tggaacaaac ggtcggctcc ccagacacat ccattttccg
      121 ggatatttgt ggaagatgga gtagagtcta cccatacacc ggaaagggca tccaacaaag
      181 catcgcgtat gtccccgctt ttatgttctt caccaacaga ttgtgccagc ccctttaagg
      241 tgacgtatgg atttgtccag tacgccattt gtttgtcttt aaaccaaagt ataacttccg
      301 gtactggaca ttttgtctta accacgattc ccgatagcgc ctcgctgagg tttgataccg
      361 ggggtgccgc atagtcccac gcctcatata ccgatgacac gcacggttcc gttataatca
      421 aactcacatc cgatagcggt ttggctccaa aaaacaacgg agtgtcgtct tggagatgaa
      481 gacaatacgc gattgtgata gtttttaaaa aaactatctg cagtaaccat ttatgtgatg
      541 ccatgctagc ctatagtgag tcgtattaag tactctagcc ttaagagctg taattgaact
      601 gggagtggac acctgtggag agaaaggcaa agtggatgtc agtaagacca ataggtgcct
      661 atcagaaacg caagagtctt ctctgtctcg acaagcccag tttctattgg tctccttaaa
      721 cctgtcttgt aaccttgata cttacctgcc cagtgcctca cgaccaactt ctgcaaggct
      781 accttaagag agtcactgca gcttaagttc gagactgttg tgtcagaagc actgactgcg
      841 ttagcaattt aactgtgata aactaccgca ataaagcttc tagtgatctg acggttcact
      901 aaa
//
