LOCUS       sequence-111241-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recipient replicative vector to construct FLAG tagged fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, 
            Patron-Soberano A, De Las Penas A, Castano I
  TITLE     Expression vectors for C-terminal fusions with fluorescent proteins 
            and epitope tags in Candida glabrata.
  JOURNAL   Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020.
  PUBMED    25986172
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020."
COMMENT     Sequence Label: pYC64
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(86..109)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(116..139)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(146..169)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(216..232)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(217..233)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      complement(254..501)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     467..485
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 ttaaccctca ctaaagggaa caaaagctgg agctccaccg cggtggcggc cgctctagaa
       61 agtataggaa cttcggatct tatcacttgt catcgtcatc tttgtagtca gcccctttat
      121 cgtcatcgtc cttataatcg gctcctttgt catcgtcatc tttgtagtct gctcctagga
      181 tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct cgaggggggg
      241 cccggtaccg gccgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt
      301 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata
      361 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag
      421 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc
      481 gtgacataac taattacatg actcgactta ggggcagggc atgctcatgt agagcgcctg
      541 ctcgccgtcc gaggcggtgc cgtcgtacag ggcggtgtcc aggccgcaga gggtgaaccc
      601 catccgccgg tacgcgtgga tcgccggtgc gttgacgttg gtgacctcca gccagaggtg
      661 cccggcgccc agctcgcg
//
