LOCUS       sequence-111244-         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recipient replicative vector to construct MYC tagged fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, 
            Patron-Soberano A, De Las Penas A, Castano I
  TITLE     Expression vectors for C-terminal fusions with fluorescent proteins 
            and epitope tags in Candida glabrata.
  JOURNAL   Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020.
  PUBMED    25986172
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020."
COMMENT     Sequence Label: pYC66
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..25
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7..25
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(135..632)
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
ORIGIN
        1 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctccaccgcg gtggcggccg
       61 ctctagaaag tataggaact tcggatctgg cgcgaattca ctagctagtg attgattaat
      121 ttttgttcac cgttcaagtc ttcctcggag attagctttt gttcaccgtt caaatcttct
      181 tcagaaatca acttttgttc accgtcgagt ccgttcaagt cttcttctga gattaatttt
      241 tgttcaccgt tcaagtcttc ctcggagatt agcttttgtt caccgttcaa atcttcttca
      301 gaaatcaact tttgttcacc gtcgagtccg ttcaagtctt cttctgagat taatttttgt
      361 tcaccgttca agtcttcctc ggagattagc ttttgttcac cgttcaaatc ttcttcagaa
      421 atcaactttt gttcaccgtc gagtccgttc aagtcttctt ctgagattaa tttttgttca
      481 ccgttcaagt cttcctcgga gattagcttt tgttcaccgt tcaaatcttc ttcagaaatc
      541 aacttttgtt caccgtcgag tccgttcaag tcttcttctg agattaattt ttgttcaccg
      601 ttcaagtctt cctcggagat tagcttttgt tcaccgttaa ttaaccctag gatcccccgg
      661 gctgcaggaa ttcgatatca agcttatcga taccgtc
//
