LOCUS       sequence-111252-         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of full-length Nup88.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Lussi YC, Hugi I, Laurell E, Kutay U, Fahrenkrog B
  TITLE     The nucleoporin Nup88 is interacting with nuclear lamin A.
  JOURNAL   Mol Biol Cell. 2011 Apr;22(7):1080-90. doi: 10.1091/mbc.E10-05-0463.
            Epub 2011 Feb 2.
  PUBMED    21289091
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E10-05-0463"; journalName: "Mol Biol Cell"; date: 
            "2011-04"; volume: "22"; issue: "7"; pages: "1080-90"
COMMENT     Sequence Label: pcDNA3.1-Nup88
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..89
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             105..122
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(145..162)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    151..375
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(508..527)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tggtgaagca aatcaatgat atccgcaatc atgtaaactt cggctctaga gggcccttcg
       61 aacaaaaact catctcagaa gaggatctga atatgcatac cggtcatcat caccatcacc
      121 attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
      181 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
      241 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
      301 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
      361 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
      421 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      481 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      541 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      601 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagt
//
