LOCUS       sequence-111254-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with Citrine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_Citrine
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(27..737,738..740,741..743))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(69..90)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     418..437
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     677..698
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcgcacattt ccccgaattg gtcgaccttg tacagctcgt ccatgccgag agtgatcccg
       61 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
      121 agggcggact ggtagctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
      181 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
      241 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
      301 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      361 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
      421 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
      481 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa gcacatcagg
      541 ccgtagccga aggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
      601 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
      661 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
      721 agctcctcgc ccttgctcac catctcgagt tacgcgtttg atatcatggc tcactgact
//
