LOCUS       sequence-111256-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_mCherry
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..747)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(315..334)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     584..602
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttccgcgcac atttccccga attggtcgac atctgtagac ttgtacagct cgtccatgcc
       61 gccggtggag tggcggccct cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt
      121 gtgggaggtg atgtccaact tgatgttgac gttgtaggcg ccgggcagct gcacgggctt
      181 cttggccttg taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag
      241 cctctgcttg atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc
      301 ctcccagccc atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg
      361 cagcttcacc ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac
      421 cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag
      481 cttcaagtag tcgggaatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa
      541 ctgaggggac aggatgtccc aggcgaaggg cagggggcca cccttggtca ccttcagctt
      601 ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg
      661 gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aactccttga tgatggccat
      721 gttatcctcc tcgcccttgc tcaccatctc gagttacgcg tttgatatca tggctcactg
      781 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactc
//
