LOCUS       sequence-111259-        4540 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast ORF Nog2 in the Gateway entry vector pDONR221.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   McCann KL, Charette JM, Vincent NG, Baserga SJ
  TITLE     A protein interaction map of the LSU processome.
  JOURNAL   Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. 
            Epub 2015 Apr 15.
  PUBMED    25877921
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.256370.114"; journalName: "Genes Dev"; date: 
            "2015-04-15- 15"; volume: "29"; issue: "8"; pages: "862-75"
COMMENT     Sequence Label: pDONR221 Nog2
FEATURES             Location/Qualifiers
     source          1..4540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2665..2764)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2781..2800)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2782..2800)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2805..2821)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2805..2821)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2923..2942)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2934..3743
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3001..3020)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3890..4478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4379..4398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctac aaaatgggta ctggtaagaa agagaaatct agacgtattc gtgaaggtga
      721 caccaaagat ggtaatcttc gtgtgaaggg tgaaaatttc tacagagact ccaagagagt
      781 caaattcctg aatatgtaca ctagtgggaa ggagatcagg aataagaaag gtaatttaat
      841 tagggccgct tctttccagg actccacaat accggatgcg agggtccaac cagatcgtcg
      901 ttggttcggt aacacaagag tgatatctca ggacgcactg cagcatttca gaagtgcatt
      961 gggtgaaacc caaaaagata catatcaagt tcttctgaga agaaataagc ttcccatgtc
     1021 attgttggaa gaaaaggacg cagatgaatc cccaaaagcc agaattttgg ataccgaaag
     1081 ttatgctgat gcgtttgggc ccaaagccca aagaaagaga ccacgtcttg ctgcatccaa
     1141 tctagaggac ttggtcaagg ctacaaatga agacattacc aagtatgagg aaaagcaagt
     1201 cttagatgcc acattaggac taatggggaa ccaggaagac aaagaaaatg ggtggacctc
     1261 cgcagcaaaa gaagctattt tcagtaaggg tcaatccaaa cgtatttgga acgaattata
     1321 taaagtcatc gattcgtctg acgtggtaat acatgttcta gatgcaaggg atccattggg
     1381 tacccgttgt aagtccgtgg aagagtatat gaagaaggaa acaccacaca agcatttaat
     1441 ttatgttctt aataagtgtg atttggtacc tacctgggtt gcagtatgtt aacttttttt
     1501 tttttttttt tgattctttc caaccaagag gatagcacga gagatttttt ttttcgatag
     1561 agtatgattt ttattaccaa acctttttgt cagggtgctt ctctatccgt tttaggataa
     1621 acttatctac agaagtgtct gttactgttt tggaagagta atactgttcg cttatccata
     1681 tttccccgtt ctggggaagc ctcatgggta aattagaagg acaacctttg ttttaaaggt
     1741 ataccttcgc tttttagaac agcgaggatc ttatgagttg agcttttgtt atttgagact
     1801 ttatctcggg ctccatacaa tatgttcgta gtaaagatcc tcacaatttt ctactttttt
     1861 ttttttttta gtttaatcca ctcttcgaaa caatccatct cggctataac aatgattatt
     1921 acattctctt ggggcaaaag attacctatt atgaaacaaa atgtattttt tactaacaga
     1981 aggttttttt ttttcaattc taactttaca agtaggcagc ttgggtcaaa catttgtcaa
     2041 aggaacgtcc aactttggcg tttcacgcat ctattaccaa ctctttcggt aaaggttcgt
     2101 taattcagtt gctacgtcag ttctcacagt tgcatactga tagaaagcaa atatctgtag
     2161 ggtttatcgg ctatccaaac actggtaaat cgtccatcat taacacattg agaaagaaaa
     2221 aggtgtgtca agttgcacca atccctggtg aaactaaggt ctggcaatat attactctta
     2281 tgaaaagaat ttttttgatt gattgtccag gtattgtgcc gccttctagt aaagactccg
     2341 aagaagacat tttattcaga ggtgttgtca gagtcgaaca cgtcacacac ccagaacaat
     2401 atattccagg tgttttaaaa cgttgtcagg taaagcactt ggaaagaact tacgagatct
     2461 caggatggaa ggatgccacg gagtttattg aaattctggc aaggaaacaa ggtagattac
     2521 tgaagggtgg tgagcctgat gagtctggtg tttctaagca aatattgaat gattttaata
     2581 gaggtaaaat cccttggttc gtccttccac ctgaaaagga aggggaagaa aaaccaaaga
     2641 agaaagaagt tgagaagacg gcaaacccag ctttcttgta caaagttggc attataagaa
     2701 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     2761 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     2821 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaataatat
     2881 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
     2941 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
     3001 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
     3061 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     3121 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     3181 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
     3241 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     3301 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     3361 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     3421 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
     3481 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     3541 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     3601 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     3661 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     3721 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca
     3781 ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg
     3841 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3901 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3961 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4021 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4081 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4141 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4201 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4261 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4321 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4381 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4441 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4501 tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
