LOCUS       sequence-111260-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with PGK::tTA2-bGHpA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_PGK::tTA2-bGHpA
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        20..519
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(55..78)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     452..471
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 cgcgtaactc gagtctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
       61 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
      121 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
      181 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
      241 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
      301 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
      361 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
      421 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
      481 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cgtcgagtcg ccaccatgtc
      541 tagactggac aagagcaaag tcataaactc tgctctggaa ttactcaatg aagtcggtat
      601 cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta
      661 ctggcacgtg aagaacaagc gggccctgct cgatgccctg gcaatcgaga tgctggacag
      721 gcatcatacc cacttctg
//
