LOCUS       sequence-111262-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with PGK::tTA2-bGHpA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_PGK::tTA2-bGHpA
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    12..236
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(252..362)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
ORIGIN
        1 gaattggtcg accatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc
       61 ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca
      121 atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt
      181 caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag gcacagctcg
      241 actcatccgg ggagcatgtc aaggtcaaaa tcgtcaagag cgtcagcagg cagcatatca
      301 aggtcaaagt cgtcaagggc atcggctggg agcatgtcta agtcaaaatc gtcaagggcg
      361 tcggccggcc cgccgctttc gcactttagc tgtttctcca ggccacatat gattagttcc
      421 aggccgaaaa ggaaggcagg ttcggctccc tgatggtcga acagctcaat tgcttgtctc
      481 agaagtgggg gcatagaatc ggtggtaggt gtctctcttt cctcttttgc tacttgatgc
      541 tcctgatcct ccaatacgca gcccagtgta aagtggccca cggcggacag agcgtacagt
      601 gcgttctcca gggagaagcc ttgctgacac aggaacgcga gctgattttc cagggtttcg
      661 tactgtttct ctgttgggcg ggtgccgaga tgcactttag ccccgtcgcg atgtgagagg
      721 agagcacagc ggaatgactt ggcg
//
