LOCUS       sequence-111267-        4605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast ORF Nop4 in the Gateway entry vector pDONR221.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   McCann KL, Charette JM, Vincent NG, Baserga SJ
  TITLE     A protein interaction map of the LSU processome.
  JOURNAL   Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. 
            Epub 2015 Apr 15.
  PUBMED    25877921
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.256370.114"; journalName: "Genes Dev"; date: 
            "2015-04-15- 15"; volume: "29"; issue: "8"; pages: "862-75"
COMMENT     Sequence Label: pDONR221 Nop4
FEATURES             Location/Qualifiers
     source          1..4605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2730..2829)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2846..2865)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2847..2865)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2870..2886)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2870..2886)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2988..3007)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2999..3808
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3066..3085)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3955..4543
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4444..4463
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctac aaaatggaag aaacaataga aaatgttgaa gtgccttcta gtaatgtctc
      721 aaaacaaaat gacgacggcc tagatatgaa gactttgttt gtgaggtcta taccccagga
      781 tgtcacagat gagcagctgg cagatttctt ttccaatttt gctccgatca agcatgcggt
      841 agttgttaag gacacgaata aaaggtccag gggcttcggg ttcgttagtt ttgctgtaga
      901 ggatgacaca aaagaagccc tagctaaggc cagaaagacc aaatttaacg gacatatctt
      961 gagggtagac attgctaaaa gaagagatcg ttcaaagaaa acatcagaag tggttgaaaa
     1021 gagcacacct gagtcatctg agaaaataac gggccagaac aatgaagatg aggacgatgc
     1081 tgatggtgaa gactccatgc taaaaggtaa accgaaatta attatcagaa atatgccatg
     1141 gagttgtcgt gatccagtta aattgaaaaa gatctttggt agatatggta ccgtcgtgga
     1201 ggctaccatt ccaaggaagc gtgatggtaa attgtgtgga tttgcgtttg ttactatgaa
     1261 gaaaatcagt aattgtagaa ttgctttgga aaacaccaaa gacttgaaaa ttgatggtag
     1321 aaaggtggct gttgattttg ctgttcaaaa aaacagatgg gaagattaca aaaaagccca
     1381 gccagaaatg aatgacaaag atgataacga gtcaggaaat gaagatgctg aagaaaacca
     1441 tgacgacgaa gaagatgaaa atgaagagga agatagacaa gtagatcaag cttcaaaaaa
     1501 caaagaatcg aaacgtaaag cacaaaacaa aagggaagat ttttctgttt ttgttcgtaa
     1561 cgtcccttat gatgccactg aagaatcttt ggctccgcac ttcagtaaat ttggcagcgt
     1621 taaatacgca ttacctgtta tcgataagtc tacggggttg gctaaaggta cggcctttgt
     1681 agccttcaaa gaccaatata catataacga gtgtatcaaa aatgcgcctg cggctggctc
     1741 tacctcttta ttgataggtg atgatgtcat gcctgagtat gtgtatgaag gtcgtgttct
     1801 gtccattact ccaacgttag taagagagga tgctggaaga atggcagaaa agaacgccgc
     1861 caaaagaaag gaggccctag gtaaagcgcc aggcgaaaaa gatagacgta acttatattt
     1921 gctgaatgaa ggtagagttg ttgaaggctc aaaaatggct gatttgttaa ccaacactga
     1981 catggagatc agagaaaaat cttacaaatt aagagtggag caactgaaaa agaacccatc
     2041 tttgcatttg tcgatgacta ggttggctat cagaaatctt cctagagcaa tgaatgataa
     2101 agcattaaag gcgttagctc gtaaagctgt cgtggaattt gctacagaag ttaaaaataa
     2161 ggaaagacat ccattgagta aagaagagat tatcagatct acaaaggaaa aatacaagtt
     2221 catgggtcca gatgagattg aagctcaaaa aaagaaagac aaaaaaagtg gtgttgtcaa
     2281 gcaagcaaaa gttataatgg aagttaaagg atcaactgcg ggaagaagta gaggttacgg
     2341 ttttgtagaa ttcagagatc ataaaaacgc tttgatgggc ttaaggtggt tgaattgtca
     2401 tgccgttacc tccgatgaaa ttttagaggg tttgaatgat gatgaaaaga aacaagtaga
     2461 caatgactta ggcaaaggaa gacgtctttg tgttgaattt gcgattgaga actctaacgt
     2521 cgtaaagaga agaagggaac aattgaagca agcaagaacc aagagaacaa gaccagataa
     2581 tgaagatact ggagatgtcg gagaatccga gaataagaag ccaaaaaaag aagaagcaac
     2641 gacgcccacc aatcccgatg acaaaaagat gggcgatgat ataaagagaa taatcgggtt
     2701 caagcgtaag agaaagcacg caaaaaaaaa cccagctttc ttgtacaaag ttggcattat
     2761 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     2821 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     2881 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaat
     2941 aatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     3001 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
     3061 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     3121 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     3181 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     3241 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     3301 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     3361 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     3421 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     3481 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     3541 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     3601 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     3661 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     3721 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     3781 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     3841 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag
     3901 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3961 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4021 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4081 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4141 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4201 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4261 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4321 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4381 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4441 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4501 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4561 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
