LOCUS       sequence-111269-        1120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses beta-tubulin labeled with Halo-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Uno SN, Kamiya M, Yoshihara T, Sugawara K, Okabe K, Tarhan MC, 
            Fujita H, Funatsu T, Okada Y, Tobita S, Urano Y
  TITLE     A spontaneously blinking fluorophore based on intramolecular 
            spirocyclization for live-cell super-resolution imaging.
  JOURNAL   Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1038/nchem.2002. Epub 2014 
            Jul 20.
  PUBMED    25054937
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchem.2002"; journalName: "Nat Chem"; date: "2014-08"; 
            volume: "6"; issue: "8"; pages: "681-9"
COMMENT     Sequence Label: TUBB5-Halo
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(148..1032)
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatct agagtcgcgg ccgcttagcc ggaaatctcg agcgtggaca gccagcgcgc
      181 gatctcgctg ccgatcaggt ccgggttgtc ttcttgcagc agattcagac ccgggccgat
      241 gtccacagcc ttgcagttag gcaggctttt ggccaggcga gcggcttcgg ccggtgggat
      301 cagaacgcct ggggtgcccc agaacagcag cttcgggaca ggggactggt gcagccagtc
      361 catgtattct tcgaccagcg cgacgatgtt cgctggctca ccggcgattg gcagctcgtt
      421 tgggaagcgc cacagtggct cgcggtcaac aggattcagg aacggctcgc ggtaatggtc
      481 catctcgact tcagtcagcg ggcggacgac acccatcggc agcgtaccct cgataaaaac
      541 gttctgatcg atgatcagct tgcggccgac gtcggtggtg cggaaggcct ggaaggtctc
      601 gcgggcaaat tctggccatt cgtcccaggt cgggataggg cggatgaact ccataaatgc
      661 aatacctttg acgcgctctg gattgcgctt ggcccagtgg aaacccagag cggagcccca
      721 gtcgtgaatg accaggacga cctcttccag acccagggct tcgatgaagg catccatgaa
      781 gcggacgtgg tcgtcgaaga aataacccag gtctggtttg tcggatttgc ccataccgat
      841 caggtctgga gcaatgcagc gatgggtcgg tgcaacatgc gggatgatgt tgcgccacac
      901 gtaggaggag gtcgggttac cgtgcaggaa cagcacaggg gtgccatcgc gcggaccaac
      961 atcgacgtag tgcatgcgct cgcccaggac ttccacataa tgggggtcga atggaaagcc
     1021 agtaccgatt tcgctgccgg tcgactgggc ctcctcttct gcctcctctc cgaaatcctc
     1081 ttcctcttcc gcggtggcat cctggtactg ctggtactca
//
