LOCUS       sequence-111273-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  yeast ORF Tma16 in the Gateway entry vector pDONR221.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   McCann KL, Charette JM, Vincent NG, Baserga SJ
  TITLE     A protein interaction map of the LSU processome.
  JOURNAL   Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. 
            Epub 2015 Apr 15.
  PUBMED    25877921
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.256370.114"; journalName: "Genes Dev"; date: 
            "2015-04-15- 15"; volume: "29"; issue: "8"; pages: "862-75"
COMMENT     Sequence Label: pDONR221 Tma16
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5..104
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(645..744)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(761..780)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(762..780)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(785..801)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(785..801)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(903..922)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ggcccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga
       61 gcaatgcttt tttataatgc caactttgta caaaaaagca ggctacaaaa tgccggttac
      121 taaatcttta tctaagttgc aaaagaactt atccaagaaa gggaaaaata tcacggttca
      181 tccgaagggt agaaagtatg aaaagctggt cagggctact atgcgtgaag ataaaatcgc
      241 agccaaaaag aagcttcatc aagacaaaag agtccacgaa ctagctcggg taaaatttat
      301 gcaggatgta gttaactctg atacattcaa agggcaacca atatttgatc acgcccacac
      361 aagagagttt attcaaagtt ttattgaaag agatgatacg gaattagacg aactcaagaa
      421 gaagaggagg tcaaacaggc caccaagcaa tagacaagtg ctactgcaac aaaggaggga
      481 tcaagagtta aaagaattta aggcggggtt tctctgtcca gacttgagtg atgcgaagaa
      541 tatggaattt ctaagaaact ggaatggcac ttttggcctt ttgaatactc taagattgat
      601 cagaattaat gataaaggtg aacaagtcgt tggaggaaat gaaaacccag ctttcttgta
      661 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
      721 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
      781 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
      841 tgcacaagat aaaataatat catcatgaac aataaaactg tctgcttaca taaacagtaa
      901 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg agg
//
