LOCUS       sequence-111280-        1129 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For retroviral expression of E3 ubiquitin ligase osTIR1 with 9x myc 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Holland AJ, Fachinetti D, Han JS, Cleveland DW
  TITLE     Inducible, reversible system for the rapid and complete degradation 
            of proteins in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 
            10.1073/pnas.1216880109. Epub 2012 Nov 12.
  PUBMED    23150568
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1216880109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-12-4- 4"; volume: "109"; issue: "49"; pages: "E3350-7"
COMMENT     Sequence Label: pBABE TIR1-9myc
FEATURES             Location/Qualifiers
     source          1..1129
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..81)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(88..117)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(124..153)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(172..201)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(208..237)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(244..273)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(292..321)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(328..357)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(364..393)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tgtgctggcg aattcgaatg atcgttccac tttttagcta gtggatccgt tcaagtcttc
       61 ttctgagatt aatttttgtt caccgttcaa gtcttcctcg gagattagct tttgttcacc
      121 gttcaaatct tcttcagaaa tcaacttttg ttcacctcta gaggatccgt tcaagtcttc
      181 ttctgagatt aatttttgtt caccgttcaa gtcttcctcg gagattagct tttgttcacc
      241 gttcaaatct tcttcagaaa tcaacttttg ttcacctcta gaggatccgt tcaagtcttc
      301 ttctgagatt aatttttgtt caccgttcaa gtcttcctcg gagattagct tttgttcacc
      361 gttcaaatct tcttcagaaa tcaacttttg ttcaccacta gcagcagaac cggagtcgac
      421 taggatttta acaaaatttg gtgcatcatc cctcgcccca gcagttgtgc ggtacacata
      481 taatttctcc actttaggca gatctccatg gttttcctcc atttcattgc taccatcccg
      541 ctcatttatg acctcaacat tgagcatcgg catctttgac gcaaggactt ggcacccctt
      601 taacgtgaca ttgcaagatg acatccaaag ggatcgcatt gtctcgtacc tagcaaaatt
      661 ccccaagagt gcagcatcac caaacgggct atctcttatc tccagtttcc ttaaattctt
      721 gcatccattc ataacatgca tcataccctt atcactgtca ccagcaaatg ctatagaaag
      781 catctcaagt tgttttgcat atttcccaat atacatgaaa actttgtctg tgagaagacc
      841 agatattgac aaacgttgta atcccttgca ctcacgaaca atagctccaa agccttcatc
      901 taatggttgg cttgtcacaa catcaggctt ccctggctca agaatacaaa gtctgaatcg
      961 tgtgaaattt ggacagttct tggcgacagt aactagtgca gcattagtca tttggtgaca
     1021 gaagtacagt agtgagttca gttttggaca gcccaaggat actgcaacaa gtccctcctc
     1081 tgtcactgca gaataaccag ctacgtagaa atctgatgga aataccctg
//
