LOCUS       sequence-111282-        3468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast ORF Mak16 in the Gateway entry vector pDONR221.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   McCann KL, Charette JM, Vincent NG, Baserga SJ
  TITLE     A protein interaction map of the LSU processome.
  JOURNAL   Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. 
            Epub 2015 Apr 15.
  PUBMED    25877921
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.256370.114"; journalName: "Genes Dev"; date: 
            "2015-04-15- 15"; volume: "29"; issue: "8"; pages: "862-75"
COMMENT     Sequence Label: pDONR221 Mak16
FEATURES             Location/Qualifiers
     source          1..3468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1593..1692)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1709..1728)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1710..1728)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1733..1749)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1733..1749)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1851..1870)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1862..2671
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1929..1948)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2818..3406
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3307..3326
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctac aaaatgtccg acgaaattgt ttggcaagtg attaatcaaa gtttctgctc
      721 tcatagaatt aaggcaccta atggtcaaaa tttttgcaga aatgagtata acgtcactgg
      781 gttgtgtaca aggcaatcat gcccacttgc caactccaag tatgcaacag tgaagtgtga
      841 caatgggaaa ctgtacttgt atatgaagac gcctgaaaga gcgcacactc ctgccaagtt
      901 atgggaaaga atcaaactat ccaaaaatta cacgaaggct ttacaacaga tcgacgaaca
      961 cctactacat tggagcaagt ttttccgtca taagtgtaaa cagagattta caaaattgac
     1021 gcaggtcatg ataacagaga gacgtttagc attaagggag gaggagagac actacgttgg
     1081 tgtagcacca aaggtcaaaa gaagagaaca aaacagggag agaaaggcat tggtagctgc
     1141 caagattgaa aaagccatcg aaaaagaatt gatggacaga ttaaagagtg gtgcttacgg
     1201 tgataaacca ttaaatgttg acgaaaaggt ctggaagaag ataatgggtc aaatggaaga
     1261 ggagaattct caggacgagg aagaagactg ggatgaagaa gaggaaagtg atgatgggga
     1321 agttgaatac gttgcagatg atggcgaagg tgaatacgtt gacgtggatg acttagaaaa
     1381 gtggttagct gactctgaca gagaagcttc cagcgccagt gaaagcgaaa gcgacagtga
     1441 aagcgagagc gacagcgata gtgatgaaga aaataagaat tcagccaaga gacgtaagaa
     1501 gggaactagt gccaaaacta aacgtcctaa agtcgaaatc gaatacgagg aggaacacga
     1561 agttcaaaat gctgagcaag aagtggcaca aaacccagct ttcttgtaca aagttggcat
     1621 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     1681 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     1741 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     1801 aataatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     1861 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     1921 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     1981 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     2041 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     2101 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     2161 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     2221 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     2281 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     2341 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     2401 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     2461 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     2521 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     2581 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     2641 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     2701 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     2761 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2821 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2881 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2941 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3001 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3061 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3121 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3181 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3241 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3301 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3361 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3421 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
