LOCUS       sequence-111283-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal fragment of Cre fused to CRY2 for blue 
            light-induced activation of DNA recombination when used with 
            CIBN-CreC. Also expresses mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL
  TITLE     Rapid blue-light-mediated induction of protein interactions in 
            living cells.
  JOURNAL   Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524
  PUBMED    21037589
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2010-12"; volume: "7"; issue: 
            "12"; pages: "973-5"
COMMENT     Sequence Label: pmCherry-CRY2-CreN
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(564..580)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(565..581)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cgcctgtttt atactccctt cccgtaactt agacgcacaa aaccaagttc aatagaaggg
       61 ggtacaaacc agtaccacca cgaacaagca cttctgtttc cccggtgatg tcgtatagac
      121 tgcttgcgtg gttgaaagcg acggatccgt tatccgctta tgtacttcga gaagcccagt
      181 accacctcgg aatcttcgat gcgttgcgct cagcactcaa ccccagagtg tagcttaggc
      241 tgatgagtct ggacatccct caccggtgac ggtggtccag gctgcgttgg cggcctacct
      301 atggctaacg ccatgggacg ctagttgtga acaaggtgtg aagagcctat tgagctacat
      361 aagaatcctc cggcccctga atgcggctaa tcccaacctc ggagcaggtg gtcacaaacc
      421 agtgattggc ctgtcgtaac gcgcaagtcc gtggcggaac cgactacttt gggtgtccgt
      481 gtttcctttt attttattgt ggctgcttat ggtgacaatc acagattgtt atcataaagc
      541 gaattggata ggatcaagct tatcgatacc gtcgacctcg agctcgccac catgaagatg
      601 gacaaaaaga ctatagtttg gtttagaaga gacctaagga ttgaggataa tcctgcatta
      661 gcagcagctg ctcacgaagg atctgttttt cctgtcttca tttggtgtcc tgaagaagaa
      721 ggacagtttt atcctggaag agcttcaaga tggtggatga aacaatcact tgctcactta
      781 tctcaatcct tgaaggctct tggatctgac ctcactttaa tcaa
//
