LOCUS       sequence-111289-        4517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast ORF Puf6 in the Gateway entry vector pDONR221.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   McCann KL, Charette JM, Vincent NG, Baserga SJ
  TITLE     A protein interaction map of the LSU processome.
  JOURNAL   Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. 
            Epub 2015 Apr 15.
  PUBMED    25877921
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.256370.114"; journalName: "Genes Dev"; date: 
            "2015-04-15- 15"; volume: "29"; issue: "8"; pages: "862-75"
COMMENT     Sequence Label: pDONR221 Puf6
FEATURES             Location/Qualifiers
     source          1..4517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    643..667
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2642..2741)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2758..2777)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2759..2777)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2782..2798)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2782..2798)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2900..2919)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2911..3720
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2978..2997)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3867..4455
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4356..4375
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttttttgact gatagtgacc
      601 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      661 gcaggctaca aaatggcacc tttaaccaag aagacaaacg gaaaacgttc tgctaaagaa
      721 gttagtcatt cagaaaagaa attagccaaa aaacctagaa tttctattga ttcgtctgat
      781 gaagaatcag aactatccaa aaaggaagat gctgtatcct cttcatcaga tgatgatgat
      841 ttggatgatt taagtaccag tgactctgaa gcagaagaag aagctgacga attagatatt
      901 tccgatgaca gtgaggaaca tgaaaatgag aatgaagaga aagaaggaaa ggacaaatct
      961 gaaggaggtg aaaatggaaa tcacactgaa caaagaaaac tgctaaaaga aaggaaaatg
     1021 caaagaaaat ctggtaccca agtgcaacaa attaaatccg tctgggaaag attgcgtgtg
     1081 aagaccccac ctttaccaaa acagattcgt gaaaaacttt ccaacgaaat ctgggagcta
     1141 tctaaggact gtatcagtga tcttgtctta aagcatgatg cttcccgtat tgttcaaact
     1201 ttagtcaagt attcgtccaa agatcgtcgt gaacaaattg tcgatgcttt aaagggaaaa
     1261 ttttatgttc tagccacatc tgcgtacggt aagtatctat tggttaagtt actgcattat
     1321 ggttcaagaa gttcaaggca aactataata aatgaattac acggttcttt gagaaaatta
     1381 atgagacatc gtgagggtgc ctacgttgtt gaagatttat ttgttcttta tgccacacat
     1441 gaacaaagac aacaaatgat taaagagttt tggggctctg aatatgctgt atttagagaa
     1501 acccacaaag atttgaccat tgagaaagtt tgtgaaagta gcattgaaaa aaggaatatt
     1561 atcgccagaa acttgattgg taccattacc gcatcagtag aaaagggttc caccggcttt
     1621 caaattctac atgctgctat gagagaatat gtgaaaatag ccaacgaaaa ggaaatatct
     1681 gaaatgatcg aattgcttca tgaacaattc gctgaattgg tgcatactcc agaaggttca
     1741 gacgttgctt gtactttggt tgctagagct aacgccaagg aaagaaagtt aattttaaag
     1801 gctttgaaaa accacgccga aaaattgatc aaaaatgagt acggtaatat tgtcttcatt
     1861 acaattctga actgtgttga cgatacagtt ttagtattca agactttcag tcctactgtt
     1921 aaggaacatt tgcaagaatt cataattgat aagtttggta gaaggccttg gttatacata
     1981 ctattaggtt tggacggtaa gtatttctca cctatcgtca agaatgaatt attgagatac
     2041 attgaactgt ccaaagccac ctctaagaag gatcctttac aaagaaggca cgaacttttg
     2101 tcaaaattcg cccccatgtt tttaagcact atatcaaaag attattccag tatattgaca
     2161 gagaatctag gctgtcagtt cattgccgaa gttctaataa acgatgagtt gtacgcgcag
     2221 ctgaatgaaa aagaccaaga aaagtatcaa caggtcttaa acaacatttt aactactttt
     2281 aagggtgaca taaccgaaga agagcaccca atacatagag cattttctac tagactatta
     2341 aaggcactaa ttcaaggtgg taaatggaat aacaaagaaa aaaaggtcat accattaaaa
     2401 aatgttcaag gcttaggcgt tccatttgct gaaaagttat acgatgaaat tattgactcc
     2461 tccaacttgt tagaatggat taataacgct gatagttcat ttacaattgt tgcactgtat
     2521 gaaacgttaa aagatcaaaa agaagggaaa ccatttttga aggatttgag aggagttcag
     2581 agtaaaatca ccactgatga aagtaacaaa ggctctcagc ttttggctaa attgttaaaa
     2641 aacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     2701 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     2761 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     2821 aatctctgat gttacattgc acaagataaa ataatatcat catgaacaat aaaactgtct
     2881 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     2941 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     3001 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     3061 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     3121 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     3181 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     3241 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     3301 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     3361 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     3421 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     3481 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     3541 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     3601 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     3661 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     3721 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     3781 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     3841 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3901 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3961 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     4021 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4081 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4141 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4201 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4261 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4321 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4381 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4441 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4501 ccttttgctc acatgtt
//
