LOCUS       sequence-111301-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM
  TITLE     Linear DNA for rapid prototyping of synthetic biological circuits in
            an Escherichia coli based TX-TL cell-free system.
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. 
            Epub 2013 Dec 4.
  PUBMED    24303785
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400131a";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "387-97"
COMMENT     Sequence Label: pBADmod1-linker2-gamS
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(369..653)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(427..446)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gagaccttaa ttcagtggtg gtggtggtgg tgctcgagta cctctgaatc aatatcaacc
       61 tggtggtgag caatggtttc aaccatgtac cggatgtgtt ctgccatgcg ctcctgaaac
      121 tcaacatcgt catcaaacgc acgggtaatg gattttttgc tggccccgtg gcgttgcaaa
      181 tgatcgatgc atagcgattc aaacaggtgc tggggcaggc ctttttccat gtcgtctgcc
      241 agttctgcct ctttctcttc acgggcgagc tgctggtagt gacgcgccca gctctgagcc
      301 tcaagacgat cctgaatgta ataagcgttt cccatggtat atctccttct taaagttaaa
      361 aaacgggtat ggagaaacag tagagagttg cgataaaaag cgtcaggtag gatccgctaa
      421 tcttatggat aaaaatgcta tggcatagca aagtgtgacg ccgtgcaaat aatcaatgtg
      481 gacttttctg ccgtgattat agacactttt gttacgcgtt tttgtcatgg ctttggtccc
      541 gctttgttac agaatgcttt taataagcgg ggttaccggt ttggttagcg agaagagcca
      601 gtaaaagacg cagtgacggc aatgtctgat gcaatatgga caattggttt cttctctgaa
      661 tggcgggagt atgaaaagta tggctgaagc gcaaaatgat cccctgctgc cgggatactc
      721 gtttaatgcc catctggtgg cgggtttaac gccgattgag gccaacggtt atctcgattt
      781 ttttatcgac cgaccgctgg gaatgaaagg ttatattctc aatctcacca ttcgcggtca
      841 ggggg
//
