LOCUS       sequence-111307-        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control gRNA (GTCAAGGCACTCTTGCCTA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Moore R, Spinhirne A, Lai MJ, Preisser S, Li Y, Kang T, Bleris L
  TITLE     CRISPR-based self-cleaving mechanism for controllable gene delivery 
            in human cells.
  JOURNAL   Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 
            10.1093/nar/gku1326. Epub 2014 Dec 18.
  PUBMED    25527740
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1326"; journalName: "Nucleic Acids Res"; date: 
            "2015-01-30- 30"; volume: "43"; issue: "2"; pages: "1297-303"
COMMENT     Sequence Label: pCas9–mKate2ps–CgRNA
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    343..367
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(379..454)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(475..723)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(533..552)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(703..723)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     polyA_signal    816..863
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     888..907
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
       61 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
      121 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg
      181 cagggcctgc cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt
      241 ggggtgggga aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtgggatcc
      301 actagtaacg gccgccagtg tgctggaatt cgccctttaa tgccaacttt gtacaagaaa
      361 gctgggtcta gaaaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag
      421 ccttatttta acttgctatt tctagctcta aaactaggca agagtgcctt gaccggtgtt
      481 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggtaag
      541 catatgatag tccattttaa aacataattt taaaactgca aactacccaa gaaattatta
      601 ctttctacgt cacgtatttt gtactaatat ctttgtgttt acagtcaaat taattctaat
      661 tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg gaaataggcc
      721 ctcttcctgc ccgaccttgg taccggatcc agtcgactga ggtctcggtg gggtatcgac
      781 agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa caccgtgcgt
      841 tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg tctccttccg
      901 tgtttcagtt agcctccccc tagggtgggc gaagaactcc agcatgagat ccccgcgctg
      961 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1021 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1081 ccc
//
