LOCUS       sequence-111308-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     Ghosh Lab Plasmids
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRK5-HA-LMO4 (L4)
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(576..602)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      605..614
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(699..718)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(731..749)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(732..749)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 agttgggcca tggcggccaa gctggggatc cttacttact tagcggccgc gcagaccttc
       61 tggtctggca gtagtggatt gctctgaagt gagttcaaat ggccgttgat gagagctgtg
      121 ggtctatcat gttcacaaaa taaactgcca ttgatgtagt gaaaccgatc tcccgggact
      181 aggcgattcc ggcaggtaga gcatgtaaaa cacttgagat gatacacgtt gccttgagcc
      241 ctcatgacga gttcactcgc aggaatcgac tgtccacagg cactgcaggc accactgttc
      301 ccaaataacc taatgtagtc atttctgcaa aggatcatgc cgctcttggt gtaacaggac
      361 gtgccgatgt cgcccagctg cgcctggcag caggagcact tgaggcagcg gctgtgccag
      421 tagctgtcca tggcatagag cagaaagcgg tccgcaatct tgcccccgca gcctgcgcag
      481 cgcttccagg agagggagcc ggccgtcacc gggggcggct gcgagctgct gcccggattc
      541 accatggtcg acccaccacc accaccattg agatctgcgt agtcaggcac atcatagggg
      601 tagcccatgg tggcgcgaat ggccttggtg aagagaccga ctgtcggttc gggaattcaa
      661 tcgatagaac cgaggtgcag ttggacctgg gagtggacac ctgtggagag aaaggcaaag
      721 tggatgttat tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt
      781 aattgtagcc gcgttctaac gaagccaagg gggtgggcct atagactcta taggcggtac
      841 ttacgtcact cttggcacgg ggaatccgcg ttccaatgca ccgttcccgg ccgcgga
//
