LOCUS       sequence-111316-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS 
            controlled by TDH3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pTDH3-dCas9
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cccgcattcc tgtcaggcga acaaaagaag gcaattgtgg accttctgtt caagaccaat
       61 agaaaggtga ccgtgaagca gctgaaggag gactatttca agaaaattga atgcttcgac
      121 tctgtggaga ttagcggggt cgaagatcgg ttcaacgcaa gcctgggtac ctaccatgat
      181 ctgcttaaga tcatcaagga caaggatttt ctggacaatg aggagaaaga ggacatcctt
      241 gaggacattg tcctgactct cactctgttc gaggaccggg aaatgatcga ggagaggctt
      301 aagacctacg cccatctgtt cgacgataaa gtgatgaagc aacttaaacg gagaagatat
      361 accggatggg gacgccttag ccgcaaactc atcaacggaa tccgggacaa acagagcgga
      421 aagaccattc ttgatttcct taagagcgac ggattcgcta atcgcaactt catgcaactt
      481 atccatgatg attccctgac ctttaaggag gacatccaga aggcccaagt gtctggacaa
      541 ggtgactcac tgcacgagca tatcgcaaat ctggctggtt cacccgctat taagaagggt
      601 attctccaga ccgtgaaagt cgtggacgag ctggtcaagg tgatgggtcg ccataaacca
      661 gagaacattg tcatcgagat ggccagggaa aaccagacta cccagaaggg acagaagaac
      721 agcagggagc ggatgaaaag aattgaggaa gggattaagg agctcgggtc acagatcctt
      781 aaagagcacc cggtggaaaa cacccagctt cagaatgaga agctctatct gtactacctt
      841 caaaatggac gcgatatgta tgtggaccaa gagcttgata tcaacaggct ctcagactac
      901 gacgtggacg ccatcgtcc
//
