LOCUS       sequence-111317-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  yeast ORF Nip7 in the Gateway entry vector pDONR221.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   McCann KL, Charette JM, Vincent NG, Baserga SJ
  TITLE     A protein interaction map of the LSU processome.
  JOURNAL   Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. 
            Epub 2015 Apr 15.
  PUBMED    25877921
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.256370.114"; journalName: "Genes Dev"; date: 
            "2015-04-15- 15"; volume: "29"; issue: "8"; pages: "862-75"
COMMENT     Sequence Label: pDONR221 Nip7
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    75..99
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(649..748)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(765..784)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(766..784)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(789..805)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(789..805)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ccaatatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat
       61 gcttttttat aatgccaact ttgtacaaaa aagcaggcta caaaatgaga cagctaacag
      121 aagaagagac caaggttgtt ttcgaaaaac tcgccggcta tattggtaga aatatttctt
      181 ttttagtgga taataaagaa cttcctcatg tcttcagatt acaaaaggat agagtatatt
      241 acgtacccga tcatgttgca aaattggcta ctagtgtagc aagacctaac ttgatgtctc
      301 tgggtatttg tttaggtaaa ttcacgaaaa ctggaaaatt caggttacat attacttctt
      361 tgacagtatt ggctaagcat gccaaatata agatatggat taaacctaat ggggagatgc
      421 cattcttata cggtaatcat gtgttaaagg cacatgtggg taaaatgtct gatgatatac
      481 cagaacacgc cggtgttatt gtgtttgcaa tgaatgatgt gccattaggg ttcggtgtca
      541 gtgcgaaaag tacttctgag tcaagaaaca tgcaacccac tggtatagtt gctttcagac
      601 aagcagatat tggtgagtat ttgagagatg aagacacctt gtttactaac ccagctttct
      661 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
      721 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt
      781 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
      841 acattgcaca agataaaata atatcatcat gaacaataaa actgtctgct tacataaaca
      901 gtaatacaag gggtgttatg agcc
//
