LOCUS       sequence-111318-       11057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS 
            controlled by TDH3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 11057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pTDH3-dCas9
FEATURES             Location/Qualifiers
     source          1..11057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     30..52
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     44..61
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     45..61
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     68..87
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        68..86
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        136..802
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     672..695
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     regulatory      831..840
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4950..4970
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4998..5185
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5053..5077)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        complement(5263..5281)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5302..5318)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5302..5318)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5315..5337)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5326..5342
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5350..5356,5357..5374,5375..5380))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(5533..5550)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5704..6292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(6463..7254,7255..7323))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7086..7105
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7324..7428)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    7465..7968
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     8009..8027
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8065..8087)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8187..8206
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(8700..9794)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(9795..10202)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(10502..10957)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10639..10660)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10851..10870
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
       61 tgaattgtaa tacgactcac tatagggcga attggagctc actcggaatt cagtctacta
      121 gtcagttcga gtttatcatt atcaatactg ccatttcaaa gaatacgtaa ataattaata
      181 gtagtgattt tcctaacttt atttagtcaa aaaattagcc ttttaattct gctgtaaccc
      241 gtacatgccc aaaatagggg gcgggttaca cagaatatat aacatcgtag gtgtctgggt
      301 gaacagttta ttcctggcat ccactaaata taatggagcc cgctttttaa gctggcatcc
      361 agaaaaaaaa agaatcccag caccaaaata ttgttttctt caccaaccat cagttcatag
      421 gtccattctc ttagcgcaac tacagagaac aggggcacaa acaggcaaaa aacgggcaca
      481 acctcaatgg agtgatgcaa cctgcctgga gtaaatgatg acacaaggca attgacccac
      541 gcatgtatct atctcatttt cttacacctt ctattacctt ctgctctctc tgatttggaa
      601 aaagctgaaa aaaaaggttg aaaccagttc cctgaaatta ttcccctact tgactaataa
      661 gtatataaag acggtaggta ttgattgtaa ttctgtaaat ctatttctta aacttcttaa
      721 attctacttt tatagttagt ctttttttta gttttaaaac accaagaact tagtttcgaa
      781 taaacacaca taaacaaaca aaagatctga tatggatcga attagatctc gccaccatgg
      841 acaagaagta ttctatcgga ctggccatcg ggactaatag cgtcgggtgg gccgtgatca
      901 ctgacgagta caaggtgccc tctaagaagt tcaaggtgct cgggaacacc gaccggcatt
      961 ccatcaagaa aaatctgatc ggagctctcc tctttgattc aggggagacc gctgaagcaa
     1021 cccgcctcaa gcggactgct agacggcggt acaccaggag gaagaaccgg atttgttacc
     1081 ttcaagagat attctccaac gaaatggcaa aggtcgacga cagcttcttc cataggctgg
     1141 aagaatcatt cctcgtggaa gaggataaga agcatgaacg gcatcccatc ttcggtaata
     1201 tcgtcgacga ggtggcctat cacgagaaat acccaaccat ctaccatctt cgcaaaaagc
     1261 tggtggactc aaccgacaag gcagacctcc ggcttatcta cctggccctg gcccacatga
     1321 tcaagttcag aggccacttc ctgatcgagg gcgacctcaa tcctgacaat agcgatgtgg
     1381 ataaactgtt catccagctg gtgcagactt acaaccagct ctttgaagag aaccccatca
     1441 atgcaagcgg agtcgatgcc aaggccattc tgtcagcccg gctgtcaaag agccgcagac
     1501 ttgagaatct tatcgctcag ctgccgggtg aaaagaaaaa tggactgttc gggaacctga
     1561 ttgctctttc acttgggctg actcccaatt tcaagtctaa tttcgacctg gcagaggatg
     1621 ccaagctgca actgtccaag gacacctatg atgacgatct cgacaacctc ctggcccaga
     1681 tcggtgacca atacgccgac cttttccttg ctgctaagaa tctttctgac gccatcctgc
     1741 tgtctgacat tctccgcgtg aacactgaaa tcaccaaggc ccctctttca gcttcaatga
     1801 ttaagcggta tgatgagcac caccaggacc tgaccctgct taaggcactc gtccggcagc
     1861 agcttccgga gaagtacaag gaaatcttct ttgaccagtc aaagaatgga tacgccggct
     1921 acatcgacgg aggtgcctcc caagaggaat tttataagtt tatcaaacct atccttgaga
     1981 agatggacgg caccgaagag ctcctcgtga aactgaatcg ggaggatctg ctgcggaagc
     2041 agcgcacttt cgacaatggg agcattcccc accagatcca tcttggggag cttcacgcca
     2101 tccttcggcg ccaagaggac ttctacccct ttcttaagga caacagggag aagattgaga
     2161 aaattctcac tttccgcatc ccctactacg tgggacccct cgccagagga aatagccggt
     2221 ttgcttggat gaccagaaag tcagaagaaa ctatcactcc ctggaacttc gaagaggtgg
     2281 tggacaaggg agccagcgct cagtcattca tcgaacggat gactaacttc gataagaacc
     2341 tccccaatga gaaggtcctg ccgaaacatt ccctgctcta cgagtacttt accgtgtaca
     2401 acgagctgac caaggtgaaa tatgtcaccg aagggatgag gaagcccgca ttcctgtcag
     2461 gcgaacaaaa gaaggcaatt gtggaccttc tgttcaagac caatagaaag gtgaccgtga
     2521 agcagctgaa ggaggactat ttcaagaaaa ttgaatgctt cgactctgtg gagattagcg
     2581 gggtcgaaga tcggttcaac gcaagcctgg gtacctacca tgatctgctt aagatcatca
     2641 aggacaagga ttttctggac aatgaggaga aagaggacat ccttgaggac attgtcctga
     2701 ctctcactct gttcgaggac cgggaaatga tcgaggagag gcttaagacc tacgcccatc
     2761 tgttcgacga taaagtgatg aagcaactta aacggagaag atataccgga tggggacgcc
     2821 ttagccgcaa actcatcaac ggaatccggg acaaacagag cggaaagacc attcttgatt
     2881 tccttaagag cgacggattc gctaatcgca acttcatgca acttatccat gatgattccc
     2941 tgacctttaa ggaggacatc cagaaggccc aagtgtctgg acaaggtgac tcactgcacg
     3001 agcatatcgc aaatctggct ggttcacccg ctattaagaa gggtattctc cagaccgtga
     3061 aagtcgtgga cgagctggtc aaggtgatgg gtcgccataa accagagaac attgtcatcg
     3121 agatggccag ggaaaaccag actacccaga agggacagaa gaacagcagg gagcggatga
     3181 aaagaattga ggaagggatt aaggagctcg ggtcacagat ccttaaagag cacccggtgg
     3241 aaaacaccca gcttcagaat gagaagctct atctgtacta ccttcaaaat ggacgcgata
     3301 tgtatgtgga ccaagagctt gatatcaaca ggctctcaga ctacgacgtg gacgccatcg
     3361 tccctcagag cttcctcaaa gacgactcaa ttgacaataa ggtgctgact cgctcagaca
     3421 agaaccgggg aaagtcagat aacgtgccct cagaggaagt cgtgaaaaag atgaagaact
     3481 attggcgcca gcttctgaac gcaaagctga tcactcagcg gaagttcgac aatctcacta
     3541 aggctgagag gggcggactg agcgaactgg acaaagcagg attcattaaa cggcaacttg
     3601 tggagactcg gcagattact aaacatgtag cccaaatcct tgactcacgc atgaatacca
     3661 agtacgacga aaacgacaaa cttatccgcg aggtgaaggt gattaccctg aagtccaagc
     3721 tggtcagcga tttcagaaag gactttcaat tctacaaagt gcgggagatc aataactatc
     3781 atcatgctca tgacgcatat ctgaatgccg tggtgggaac cgccctgatc aagaagtacc
     3841 caaagctgga aagcgagttc gtgtacggag actacaaggt ctacgacgtg cgcaagatga
     3901 ttgccaaatc tgagcaggag atcggaaagg ccaccgcaaa gtacttcttc tacagcaaca
     3961 tcatgaattt cttcaagacc gaaatcaccc ttgcaaacgg tgagatccgg aagaggccgc
     4021 tcatcgagac taatggggag actggcgaaa tcgtgtggga caagggcaga gatttcgcta
     4081 ccgtgcgcaa agtgctttct atgcctcaag tgaacatcgt gaagaaaacc gaggtgcaaa
     4141 ccggaggctt ttctaaggaa tcaatcctcc ccaagcgcaa ctccgacaag ctcattgcaa
     4201 ggaagaagga ttgggaccct aagaagtacg gcggattcga ttcaccaact gtggcttatt
     4261 ctgtcctggt cgtggctaag gtggaaaaag gaaagtctaa gaagctcaag agcgtgaagg
     4321 aactgctggg tatcaccatt atggagcgca gctccttcga gaagaaccca attgactttc
     4381 tcgaagccaa aggttacaag gaagtcaaga aggaccttat catcaagctc ccaaagtata
     4441 gcctgttcga actggagaat gggcggaagc ggatgctcgc ctccgctggc gaacttcaga
     4501 agggtaatga gctggctctc ccctccaagt acgtgaattt cctctacctt gcaagccatt
     4561 acgagaagct gaaggggagc cccgaggaca acgagcaaaa gcaactgttt gtggagcagc
     4621 ataagcatta tctggacgag atcattgagc agatttccga gttttctaaa cgcgtcattc
     4681 tcgctgatgc caacctcgat aaagtcctta gcgcatacaa taagcacaga gacaaaccaa
     4741 ttcgggagca ggctgagaat atcatccacc tgttcaccct caccaatctt ggtgcccctg
     4801 ccgcattcaa gtacttcgac accaccatcg accggaaacg ctatacctcc accaaagaag
     4861 tgctggacgc caccctcatc caccagagca tcaccggact ttacgaaact cggattgacc
     4921 tctcacagct cggaggggat gagggagctc ccaagaaaaa gcgcaaggta ggtagttccg
     4981 gatcctagac tctcgaggcg aatttcttat gatttatgat ttttattatt aaataagtta
     5041 taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct
     5101 tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg
     5161 ctcttattga ccacacctct accggcatgc cgagcaaatg cctgcaaatc gctccccatt
     5221 tctctagata agactgcaga gttcggtacc cagcttttgt tccctttagt gagggttaat
     5281 tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5341 aattccacac aacataggag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     5401 gaggtaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     5461 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     5521 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5581 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5641 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5701 gtttttccat aggctcggcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5761 gtggcgaaac ccgacaggac tataaagata ccaggcgttc ccccctggaa gctccctcgt
     5821 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5881 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5941 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6001 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6061 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6121 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     6181 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6241 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6301 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6361 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6421 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6481 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactgcccgt
     6541 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6601 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6661 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6721 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6781 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6841 atcaaggcga gttacatgat cccccatgtt gtgaaaaaaa gcggttagct ccttcggtcc
     6901 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6961 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7021 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     7081 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7141 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7201 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7261 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7321 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7381 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7441 aaaagtgcca cctgggtcct tttcatcacg tgctataaaa ataattataa tttaaatttt
     7501 ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt
     7561 ttttgttttc cgaagatgta aaagactcta gggggatcgc caacaaatac taccttttat
     7621 cttgctcttc ctgctctcag gtattaatgc cgaattgttt catcttgtct gtgtagaaga
     7681 ccacacacga aaatcctgtg attttacatt ttacttatcg ttaatcgaat gtatatctat
     7741 ttaatctgct tttcttgtct aataaatata tatgtaaagt acgctttttg ttgaaatttt
     7801 ttaaaccttt gtttattttt ttttcttcat tccgtaactc ttctaccttc tttatttact
     7861 ttctaaaatc caaatacaaa acataaaaat aaataaacac agagtaaatt cccaaattat
     7921 tccatcatta aaagatacga ggcgcgtgta agttacaggc aagcgatccg tctaagaaac
     7981 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     8041 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     8101 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     8161 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     8221 tatcgactac gtcgttaagg ccgtttctga cagagtaaaa ttcttgaggg aactttcacc
     8281 attatgggaa atggttcaag aaggtattga cttaaactcc atcaaatggt caggtcattg
     8341 agtgtttttt atttgttgta tttttttttt tttagagaaa atcctccaat atataaatta
     8401 ggaatcatag tttcatgatt ttctgttaca cctaactttt tgtgtggtgc cctcctcctt
     8461 gtcaatatta atgttaaagt gcaattcttt ttccttatca cgttgagcca ttagtatcaa
     8521 tttgcttacc tgtattcctt tacatcctcc tttttctcct tcttgataaa tgtatgtaga
     8581 ttgcgtatat agtttcgtct accctatgaa catattccat tttgtaattt cgtgtcgttt
     8641 ctattatgaa tttcatttat aaagtttatg tacaaatatc ataaaaaaag agaatctttt
     8701 taagcaagga ttttcttaac ttcttcggcg acagcatcac cgacttcggt ggtactgttg
     8761 gaaccaccta aatcaccagt tctgatacct gcatccaaaa cctttttaac tgcatcttca
     8821 atggccttac cttcttcagg caagttcaat gacaatttca acatcattgc agcagacaag
     8881 atagtggcga tagggttgac cttattcttt ggcaaatctg gagcagaacc gtggcatggt
     8941 tcgtacaaac caaatgcggt gttcttgtct ggcaaagagg ccaaggacgc agatggcaac
     9001 aaacccaagg aacctgggat aacggaggct tcatcggaga tgatatcacc aaacatgttg
     9061 ctggtgatta taataccatt taggtgggtt gggttcttaa ctaggatcat ggcggcagaa
     9121 tcaatcaatt gatgttgaac cttcaatgta ggaaattcgt tcttgatggt ttcctccaca
     9181 gtttttctcc ataatcttga agaggccaaa acattagctt tatccaagga ccaaataggc
     9241 aatggtggct catgttgtag ggccatgaaa gcggccattc ttgtgattct ttgcacttct
     9301 ggaacggtgt attgttcact atcccaagcg acaccatcac catcgtcttc ctttctctta
     9361 ccaaagtaaa tacctcccac taattctctg acaacaacga agtcagtacc tttagcaaat
     9421 tgtggcttga ttggagataa gtctaaaaga gagtcggatg caaagttaca tggtcttaag
     9481 ttggcgtaca attgaagttc tttacggatt tttagtaaac cttgttcagg tctaacacta
     9541 ccggtacccc atttaggacc acccacagca cctaacaaaa cggcatcagc cttcttggag
     9601 gcttccagcg cctcatctgg aagtggaaca cctgtagcat cgatagcagc accaccaatt
     9661 aaatgatttt cgaaatcgaa cttgacattg gaacgaacat cagaaatagc tttaagaacc
     9721 ttaatggctt cggctgtgat ttcttgacca acgtggtcac ctggcaaaac gacgatcttc
     9781 ttaggggcag acattagaat ggtatatcct tgaaatatat atatatatat tgctgaaatg
     9841 taaaaggtaa gaaaagttag aaagtaagac gattgctaac cacctattgg aaaaaacaat
     9901 aggtccttaa ataatattgt caacttcaag tattgtgatg caagcattta gtcatgaacg
     9961 cttctctatt ctatatgaaa agccggttcc ggcgctctca cctttccttt ttctcccaat
    10021 ttttcagttg aaaaaggtat atgcgtcagg cgacctctga aattaacaaa aaatttccag
    10081 tcatcgaatt tgattctgtg cgatagcgcc cctgtgtgtt ctcgttatgt tgaggaaaaa
    10141 aataatggtt gctaagagat tcgaactctt gcatcttacg atacctgagt attcccacag
    10201 ttaactgcgg tcaagatatt tcttgaatca ggcgccttag accgctcggc caaacaacca
    10261 attacttgtt gagaaataga gtataattat cctataaata taacgttttt gaacacacat
    10321 gaacaaggaa gtacaggaca attgattttg aagagaatgt ggattttgat gtaattgttg
    10381 ggattccatt tttaataagg caataatatt aggtatgtag atatactaga agttctcctc
    10441 gacggtcgat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    10501 gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
    10561 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
    10621 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
    10681 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
    10741 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
    10801 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
    10861 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
    10921 acacccgccg cgcttaatgc gccgctacag ggcgcgtcgc gccattcgcc attcaggctg
    10981 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaag
    11041 gggggatgtg ctgcaag
//
