LOCUS       sequence-111337-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses sgRNA under U6b promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pU6b:sgRNA#3
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(663..680)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(663..679)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(672..694)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgcgtcaccc ggcaaccttg ggtagcagcg aagtcgaggc atttctgtcc tggctggtct
       61 agaggtctct ttggtttaaa aaaagcaccg actcggtgcc actttttcaa gttgataacg
      121 gactagcctt atttaaactt gctatgctgt ttccagcata gctcttaaac agagacggtc
      181 gacagtctgc agtgtcgtct ctcgaactgg gagtctggag gacggctata tataccagtg
      241 acttctgggt atgtgtttca tgtgtggtat atggcttact tacttattca actcatttac
      301 acttacatgt gtcagtaaat cactgtgtag tagtaaacct gtaaaccctt aaattcttta
      361 aaatttgaaa accagcagtt tatgtaggtt taccgtagag ttttggagct gtaagcgtat
      421 gcgaaggaaa caggccgttt ctttagagtg attggatgag ttcctgaact tcctgtgtga
      481 catcgaggag cttttgttca cgtgtccttc tcctcccccc acaggcatgc gcagatcccc
      541 cctgccttgg ataagagcac aaaacactcc gacaaaagga agctgctctc actagagcag
      601 agcccatgaa aacccagaca gacacgtgga gggtaatgag agagaccggg cccgagctta
      661 agactggccg tcgttttaca acgtcgtgac tgggaaaaca tccatgctag cgttaacgcg
      721 agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcggaag actggg
//
