LOCUS       sequence-111344-        4403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast ORF Nop2 in the Gateway entry vector pDONR221.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   McCann KL, Charette JM, Vincent NG, Baserga SJ
  TITLE     A protein interaction map of the LSU processome.
  JOURNAL   Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. 
            Epub 2015 Apr 15.
  PUBMED    25877921
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.256370.114"; journalName: "Genes Dev"; date: 
            "2015-04-15- 15"; volume: "29"; issue: "8"; pages: "862-75"
COMMENT     Sequence Label: pDONR221 Nop2
FEATURES             Location/Qualifiers
     source          1..4403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2528..2627)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2644..2663)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2645..2663)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2668..2684)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2668..2684)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2786..2805)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2797..3606
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2864..2883)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3753..4341
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4242..4261
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctac aaatgggtag tagacgtcat aagaacaagc aagccgcccc accaaccctg
      721 gaggagtttc aggcaagaaa ggaaaagaag gctaatagaa agctagaaaa aggaaagaga
      781 ccttctacca cgcaagggga tgaagtttct gatagaaaga aaaagaagtc gaagccattc
      841 aagaaatcta gaaaagagga ggaagaagtt gttgaagagg ataaggattt gccagaagtt
      901 gatcttgaag aattatcaaa agccaggaaa tctttatttg atgatgaaga agatgacgat
      961 gaagcaggat tggtggatga agaactaaaa gatgaatttg acttggaaca agaatatgat
     1021 tacgatgaag atgaagataa tgacgcccac ccaatttttt cagatgacga cgatgaggct
     1081 gatcttgaag aactaaatgc acaaaacatg gaagctcttt ctaagaaact tgatgaagaa
     1141 gaagctgaag aagctgaaga agccgaaatg gagttagtgg aagcggagaa catgcaacca
     1201 agagcagaca tattgcccac tgaggagcag gaagaaatga tggcacaaga aactcctaat
     1261 ttaacttcca caagaacaag aatgattgaa attgtaaagg ttttggaaaa ttttaagact
     1321 cttggtgcag aaggcagatc gagaggagaa tacgttgaca gacttttgaa agatatttgt
     1381 gagtactttg gttatacacc atttttggcg gaaaagttat tcaatctatt ctcaccagca
     1441 gaggcgatgg agtttttcga agccaatgaa attgcaagac caataaccat cagaaccaac
     1501 accttgaaga ccagaaggag agacttagct caaactcttg tgaatagagg tgttaatcta
     1561 caacctattg gttcttggac taaagttggt cttcaaatat tcgattccca agtcccaatt
     1621 ggtgctacgc cagaatattt ggcaggtcac tatattttac aagcggcatc atcttttcta
     1681 ccagttattg ctctagatcc tcatgaaaat gagcgtatct tggatatggc agctgctcca
     1741 ggtggtaaga ccacttatat atcagctatg atgaaaaaca caggttgtgt tttcgctaac
     1801 gacgccaata aatctagaac aaaatcttta attgctaata tccaccgtct aggctgtacc
     1861 aacactattg tatgcaatta cgatgcccgt gagttcccta aggtaattgg aggttttgac
     1921 agaattttac tggatgcccc atgttccggt actggtgtta tcggtaagga tcaatctgtc
     1981 aaggtgtctc gtaccgagaa ggacttcatt caaattccac atctacaaaa acaattgctt
     2041 ctatccgcca ttgattctgt tgattgcaac tcgaaacatg gtggtgtaat agtatattcg
     2101 acatgttctg ttgcagtgga agaggacgaa gctgtcatag actatgcatt gagaaaaaga
     2161 ccgaatgtca agttagtcga tactggttta gcgatcggta aagaagcgtt tactagttac
     2221 agaggcaaaa agtttcatcc tagtgtgaaa ttagcgagga ggtactatcc acatacgtac
     2281 aacgtcgatg ggttctttgt cgctaaattc caaaagatcg gtccatcttc gtttgatgat
     2341 aaccaagcaa gtgcaaaaga aaaggaaact gctgctagaa aggaagcttt ggaagaaggt
     2401 atcattcact ctgacttcgc aaccttcgag gacgaagaag acgataagta cattgaaaag
     2461 tctgtgaaaa acaatctttt gaaaaagggt gtcaatccaa aagctaaaag accttctaac
     2521 gaaaaaaacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     2581 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     2641 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     2701 tctcaaaatc tctgatgtta cattgcacaa gataaaataa tatcatcatg aacaataaaa
     2761 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     2821 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     2881 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     2941 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     3001 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     3061 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     3121 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     3181 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     3241 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     3301 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     3361 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     3421 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     3481 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     3541 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     3601 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     3661 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     3721 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3781 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3841 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3901 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3961 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4021 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4081 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4141 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4201 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4261 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4321 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4381 tgctggcctt ttgctcacat gtt
//
