LOCUS       sequence-111345-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses sgRNA under U6d promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pU6d:sgRNA#5
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(629..646)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(629..645)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(638..660)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      709..779
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtgtgcgtca cccggcaacc ttgggtagca gcgaagtcga ggcatttctg tcctggctgg
       61 tctagaggtc tctgaacttt aaaaaaagca ccgactcggt gccacttttt caagttgata
      121 acggactagc cttatttaaa cttgctatgc tgtttccagc atagctctta aacagagacg
      181 gtcgacagtc tgcagtgtcg tctctcgaac tgagagccgg aagaagagct atatatacca
      241 gggaattctg ggtatgtttc ttactggtgg taagtagata caccccttat ttaattagct
      301 tccaacaaat ttgaatgtat acactggtga gatagacctg taaactctta tgatctgcaa
      361 aattgcaagc caatgttttt gtggaaggtt tattttgagt atttcctcag ctgtagacca
      421 catgtaaaaa aggttcctct ctgctagctg tgattggatg tttcggtgtg acagtgaaga
      481 acccgtggcc acaatgttcc acaccctccc ccacagacat gcgtagaatg tttccccctc
      541 aacgatgacg agaacaaaag gttgaaagac cagcaaatgc tcctttcaca accaccaaat
      601 cacaggagag accgggcccg agcttaagac tggccgtcgt tttacaacgt cgtgactggg
      661 aaaacatcca tgctagcgtt aacgcgagag tagggaactg ccaggcatca aataaaacga
      721 aaggctcagt cggaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctct
//
