LOCUS       sequence-111347-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses sgRNA under U6a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pU6a:sgRNA#1
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(638..655)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(638..654)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(647..669)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cgtcacccgg caaccttggg tagcagcgaa gtcgaggcat ttctgtcctg gctggtctag
       61 aggtctctga ctaaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag
      121 ccttatttaa acttgctatg ctgtttccag catagctctt aaacagagac ggtcgacagt
      181 ctgcagtgtc gtctctcgaa ccaagagctg gagggagagc tatatatacc agggacttct
      241 gggtatgttt ttgggaggtg gtgagtgact aaaccactta ttcagctccc ttagatcaag
      301 tctgacccta tatcatggtg acataaacct gcaaactgat aaaacctgaa ggatctcaaa
      361 tccagagttt gtgtgaggga ttaccgtggt aattttcaaa ctgtaaagca tatgcaaaat
      421 tatctggtct tggcttgagt gattgggtgt ctcggtgtga tgcagggacg ttttcagtga
      481 cgtgtccttc tccctccccc acaggcatgc gcagaacatt tcccccctcc ttgaagacca
      541 gaacaaaaga cgccgagagc aggaaactcg tcttactgaa tgaccgaggc tggagaaacg
      601 ctgagagact tactgagaga ccgggcccga gcttaagact ggccgtcgtt ttacaacgtc
      661 gtgactggga aaacatccat gctagcgtta acgcgagagt agggaactgc caggcatcaa
      721 ataaa
//
