LOCUS       sequence-111351-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses sgRNA under U6d promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pU6d:sgRNA#5
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..28
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12..28
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcacgacgt tgtaaaacga cggccagtct taagctcggg cccggtctct cctgtgattt
       61 ggtggttgtg aaaggagcat ttgctggtct ttcaaccttt tgttctcgtc atcgttgagg
      121 gggaaacatt ctacgcatgt ctgtggggga gggtgtggaa cattgtggcc acgggttctt
      181 cactgtcaca ccgaaacatc caatcacagc tagcagagag gaaccttttt tacatgtggt
      241 ctacagctga ggaaatactc aaaataaacc ttccacaaaa acattggctt gcaattttgc
      301 agatcataag agtttacagg tctatctcac cagtgtatac attcaaattt gttggaagct
      361 aattaaataa ggggtgtatc tacttaccac cagtaagaaa catacccaga attccctggt
      421 atatatagct cttcttccgg ctctcagttc gagagacgac actgcagact gtcgaccgtc
      481 tctgtttaag agctatgctg gaaacagcat agcaagttta aataaggcta gtccgttatc
      541 aacttgaaaa agtggcaccg agtcggtgct ttttttaaag ttcagagacc tctagaccag
      601 ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg
      661 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
      721 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
      781 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tt
//
