LOCUS       sequence-111353-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses sgRNA under U6a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pU6a:sgRNA#1
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..18
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2..18
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgtaaaacga cggccagtct taagctcggg cccggtctct cagtaagtct ctcagcgttt
       61 ctccagcctc ggtcattcag taagacgagt ttcctgctct cggcgtcttt tgttctggtc
      121 ttcaaggagg ggggaaatgt tctgcgcatg cctgtggggg agggagaagg acacgtcact
      181 gaaaacgtcc ctgcatcaca ccgagacacc caatcactca agccaagacc agataatttt
      241 gcatatgctt tacagtttga aaattaccac ggtaatccct cacacaaact ctggatttga
      301 gatccttcag gttttatcag tttgcaggtt tatgtcacca tgatataggg tcagacttga
      361 tctaagggag ctgaataagt ggtttagtca ctcaccacct cccaaaaaca tacccagaag
      421 tccctggtat atatagctct ccctccagct cttggttcga gagacgacac tgcagactgt
      481 cgaccgtctc tgtttaagag ctatgctgga aacagcatag caagtttaaa taaggctagt
      541 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttagtcaga gacctctaga
      601 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
      661 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
      721 aatgcaagta gcgtatgcgc tcacgcaa
//
