LOCUS       sequence-111355-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses sgRNA under U6b promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pU6b:sgRNA#3
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..27
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11..27
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc ccggtctctc tcattaccct
       61 ccacgtgtct gtctgggttt tcatgggctc tgctctagtg agagcagctt ccttttgtcg
      121 gagtgttttg tgctcttatc caaggcaggg gggatctgcg catgcctgtg gggggaggag
      181 aaggacacgt gaacaaaagc tcctcgatgt cacacaggaa gttcaggaac tcatccaatc
      241 actctaaaga aacggcctgt ttccttcgca tacgcttaca gctccaaaac tctacggtaa
      301 acctacataa actgctggtt ttcaaatttt aaagaattta agggtttaca ggtttactac
      361 tacacagtga tttactgaca catgtaagtg taaatgagtt gaataagtaa gtaagccata
      421 taccacacat gaaacacata cccagaagtc actggtatat atagccgtcc tccagactcc
      481 cagttcgaga gacgacactg cagactgtcg accgtctctg tttaagagct atgctggaaa
      541 cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
      601 ggtgcttttt ttaaaccaaa gagacctcta gaccagccag gacagaaatg cctcgacttc
      661 gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca
      721 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgca
//
