LOCUS       sequence-111358-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for five U6x:sgRNA cassettes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pGGDestTol2LC-5sgRNA
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(28..49)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(184..696,697..699,700..702))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     377..396
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     636..657
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
       61 ctcgttgggg tctttgctca gcttggactg ggtgctcagg tagtggttgt cgggcagcag
      121 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
      181 gtcctcgatg ttgtggcgga tcttgaagtt ggccttgatg ccgttcttct gcttgtcggc
      241 ggtgatatag acgttgtcgc tgatggcgtt gtactccagc ttgtgcccca ggatgttgcc
      301 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
      361 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
      421 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
      481 gcgggcgaag cactgcacgc cccaggtcag ggtggtcacg agggtgggcc agggcacggg
      541 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
      601 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
      661 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atgttgtcag acctggtaac
      721 tccttactgt aacttattgc acctgatgaa caaaagatgt gaactgaacg caagtaggag
      781 gctaatagag gctagtacag tacaaagatc cccaaatgat gtgtgccggc ctta
//
