LOCUS       sequence-111364-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for two U6x:sgRNA cassettes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pGGDestTol2LC-2sgRNA
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(107..230)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(250..266)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(250..266)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    join(343..505,506..517)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
       61 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat
      121 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
      181 gatatttata tcattttacg tttctcgttc aacttttcta tacaaagttg atagcttggc
      241 gtaatcatgg tcatagctgt ttcctgctcg agggtctcta ctgatgcatt tcatttaatg
      301 ttttttgaga ttaaaagctt aaacaagaat ctctagtttt ctttcttgct tttactttta
      361 cttccttaat actcaagtac aattttaatg gagtactttt ttacttttac tcaagtaaga
      421 ttctagccag atacttttac ttttaattga gtaaaatttt ccctaagtac ttgtactttc
      481 acttgagtaa aatttttgag tactttttac acctctgtca agaactcctg gacaaacctc
      541 tgacctgtgt ggaacagagt ggatatgggt gtctgaacag atattcacgt cttttgcaga
      601 tcagagggca tttctggtgt tgtgcaatga cctgggtcca acaggtaggc gtactcgtat
      661 ccaagagcat tgttggacag gaggtagtct ccattcctat aaagaggaag gaatggcacc
      721 atgtagtagc cgtcattgtg gccaattggt gcattggtac gtgggtagat ggaccgggg
//
