LOCUS       sequence-111365-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for five U6x:sgRNA cassettes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pGGDestTol2LC-5sgRNA
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(106..229)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(249..265)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(249..265)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    join(342..504,505..516)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
       61 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata
      121 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
      181 atatttatat cattttacgt ttctcgttca acttttctat acaaagttga tagcttggcg
      241 taatcatggt catagctgtt tcctgctcga gggtctctac tgatgcattt catttaatgt
      301 tttttgagat taaaagctta aacaagaatc tctagttttc tttcttgctt ttacttttac
      361 ttccttaata ctcaagtaca attttaatgg agtacttttt tacttttact caagtaagat
      421 tctagccaga tacttttact tttaattgag taaaattttc cctaagtact tgtactttca
      481 cttgagtaaa atttttgagt actttttaca cctctgtcaa gaactcctgg acaaacctct
      541 gacctgtgtg gaacagagtg gatatgggtg tctgaacaga tattcacgtc ttttgcagat
      601 cagagggcat ttctggtgtt gtgcaatgac ctgggtccaa caggtaggcg tactcgtatc
      661 caagagcatt gttggacagg aggtagtctc cattcctata aagaggaagg aatggcacca
      721 tgtagtagcc gtcattgtgg ccaattggtg cattggtacg tgggtagatg gaccggggag
      781 gctgatgagt tcttagccag cctttccccg tcaagctcta aatcgggg
//
