LOCUS       sequence-111368-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for four U6x:sgRNA cassettes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pGGDestTol2LC-4sgRNA
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(108..231)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(251..267)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(251..267)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    join(344..506,507..518)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
       61 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
      121 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
      181 tgatatttat atcattttac gtttctcgtt caacttttct atacaaagtt gatagcttgg
      241 cgtaatcatg gtcatagctg tttcctgctc gagggtctct actgatgcat ttcatttaat
      301 gttttttgag attaaaagct taaacaagaa tctctagttt tctttcttgc ttttactttt
      361 acttccttaa tactcaagta caattttaat ggagtacttt tttactttta ctcaagtaag
      421 attctagcca gatactttta cttttaattg agtaaaattt tccctaagta cttgtacttt
      481 cacttgagta aaatttttga gtacttttta cacctctgtc aagaactcct ggacaaacct
      541 ctgacctgtg tggaacagag tggatatggg tgtctgaaca gatattcacg tcttttgcag
      601 atcagagggc atttctggtg ttgtgcaatg acctgggtcc aacaggtagg cgtactcgta
      661 tccaagagca ttgttggaca ggaggtagtc tccattccta taaagaggaa ggaatggcac
      721 catgtagtag ccgtcattgt ggccaattgg tgcattggta cgtgggtaga tggaccgggg
      781 aggctgatga gttcttagcc agcctttccc cgtcaagctc taaatcgggg
//
