LOCUS       sequence-111370-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for three U6x:sgRNA cassettes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pGGDestTol2LC-3sgRNA
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(99..222)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(242..258)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(242..258)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    join(335..497,498..509)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
       61 gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg
      121 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
      181 tatcatttta cgtttctcgt tcaacttttc tatacaaagt tgatagcttg gcgtaatcat
      241 ggtcatagct gtttcctgct cgagggtctc tactgatgca tttcatttaa tgttttttga
      301 gattaaaagc ttaaacaaga atctctagtt ttctttcttg cttttacttt tacttcctta
      361 atactcaagt acaattttaa tggagtactt ttttactttt actcaagtaa gattctagcc
      421 agatactttt acttttaatt gagtaaaatt ttccctaagt acttgtactt tcacttgagt
      481 aaaatttttg agtacttttt acacctctgt caagaactcc tggacaaacc tctgacctgt
      541 gtggaacaga gtggatatgg gtgtctgaac agatattcac gtcttttgca gatcagaggg
      601 catttctggt gttgtgcaat gacctgggtc caacaggtag gcgtactcgt atccaagagc
      661 attgttggac aggaggtagt ctccattcct ataaagagga aggaatggca ccatgtagta
      721 gccgtcattg tggccaattg gtgcattggt acgtgggtag atggaccggg gaggctgatg
      781 agttcttagc cagcctttcc c
//
